Urinary biomarkers in acute pyelonephritis

The aim of the study is a comparative evaluation of the diagnostic value of determining of concentration of cyto-kines in the serum and urine of patients with acute pyelonephritis. Materials and Methods: The study of cytokine profile in blood serum and urine has been performed in 21 patients with ac...

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Main Authors: Popkov V.M., Dolgov А.В., Zakharova N.B., Ponukalin A.N., Varaksin N.A.
Format: Article
Language:Russian
Published: Saratov State Medical University 2013-03-01
Series:Саратовский научно-медицинский журнал
Subjects:
Online Access:http://www.ssmj.ru/system/files/2013-01_110-115.pdf
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spelling doaj-8fe95f9db7c54e029db0b7e7a8fa72ec2021-07-02T06:17:06ZrusSaratov State Medical UniversityСаратовский научно-медицинский журнал1995-00392076-25182013-03-0191110115Urinary biomarkers in acute pyelonephritisPopkov V.M.Dolgov А.В.Zakharova N.B.Ponukalin A.N.Varaksin N.A.The aim of the study is a comparative evaluation of the diagnostic value of determining of concentration of cyto-kines in the serum and urine of patients with acute pyelonephritis. Materials and Methods: The study of cytokine profile in blood serum and urine has been performed in 21 patients with acute pyelonephritis aged from 23 to 63 years. The concentration of interleukin (IL-1 p, IL-6, IL-8, IL-10) receptor antagonist, IL-1 (IL-1RA), tumor necrosis factor — alpha (TNF-a), C-reactive protein (CRP), in serum and urine of patients under the survey have been determined by the enzyme-linked immunosorbent assay (ELISA) using the appropriate reagent sets «Vector-Best» (Novosibirsk). The study of all these biomarkers in patients with acute pyelonephritis has been performed three times: at admission before antibiotic therapy (1 point); in a 5-7 days-period from the beginning of treatment (2 points); 1.5 months after completion of treatment (3 points,). Results: Before the treatment there has been an increase in levels of IL-6, IL-8, IL-1RA, TNF-a, CRP in serum. Simultaneously, the concentration of these substances in the urine has been higher. In 2 and 3-point changes the level of cytokines has been different; however, the content of them in the urine has been determined to be higher than in the serum. Conclusion: Analysis of changes in levels of cytokines and C-reactive protein in serum and urine showed that these biomarkers in acute pyelonephritis may be used as indicators of inflammatory activity. The data received suggest that the study of cytokine levels in the urine may be used to assess the severity of inflammatory changes in the renal parenchyma and in monitoring of therapy effectiveness.http://www.ssmj.ru/system/files/2013-01_110-115.pdfacute pyelonephritisC-reactive proteinproinflammatory cytokinesurine
collection DOAJ
language Russian
format Article
sources DOAJ
author Popkov V.M.
Dolgov А.В.
Zakharova N.B.
Ponukalin A.N.
Varaksin N.A.
spellingShingle Popkov V.M.
Dolgov А.В.
Zakharova N.B.
Ponukalin A.N.
Varaksin N.A.
Urinary biomarkers in acute pyelonephritis
Саратовский научно-медицинский журнал
acute pyelonephritis
C-reactive protein
proinflammatory cytokines
urine
author_facet Popkov V.M.
Dolgov А.В.
Zakharova N.B.
Ponukalin A.N.
Varaksin N.A.
author_sort Popkov V.M.
title Urinary biomarkers in acute pyelonephritis
title_short Urinary biomarkers in acute pyelonephritis
title_full Urinary biomarkers in acute pyelonephritis
title_fullStr Urinary biomarkers in acute pyelonephritis
title_full_unstemmed Urinary biomarkers in acute pyelonephritis
title_sort urinary biomarkers in acute pyelonephritis
publisher Saratov State Medical University
series Саратовский научно-медицинский журнал
issn 1995-0039
2076-2518
publishDate 2013-03-01
description The aim of the study is a comparative evaluation of the diagnostic value of determining of concentration of cyto-kines in the serum and urine of patients with acute pyelonephritis. Materials and Methods: The study of cytokine profile in blood serum and urine has been performed in 21 patients with acute pyelonephritis aged from 23 to 63 years. The concentration of interleukin (IL-1 p, IL-6, IL-8, IL-10) receptor antagonist, IL-1 (IL-1RA), tumor necrosis factor — alpha (TNF-a), C-reactive protein (CRP), in serum and urine of patients under the survey have been determined by the enzyme-linked immunosorbent assay (ELISA) using the appropriate reagent sets «Vector-Best» (Novosibirsk). The study of all these biomarkers in patients with acute pyelonephritis has been performed three times: at admission before antibiotic therapy (1 point); in a 5-7 days-period from the beginning of treatment (2 points); 1.5 months after completion of treatment (3 points,). Results: Before the treatment there has been an increase in levels of IL-6, IL-8, IL-1RA, TNF-a, CRP in serum. Simultaneously, the concentration of these substances in the urine has been higher. In 2 and 3-point changes the level of cytokines has been different; however, the content of them in the urine has been determined to be higher than in the serum. Conclusion: Analysis of changes in levels of cytokines and C-reactive protein in serum and urine showed that these biomarkers in acute pyelonephritis may be used as indicators of inflammatory activity. The data received suggest that the study of cytokine levels in the urine may be used to assess the severity of inflammatory changes in the renal parenchyma and in monitoring of therapy effectiveness.
topic acute pyelonephritis
C-reactive protein
proinflammatory cytokines
urine
url http://www.ssmj.ru/system/files/2013-01_110-115.pdf
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AT zakharovanb urinarybiomarkersinacutepyelonephritis
AT ponukalinan urinarybiomarkersinacutepyelonephritis
AT varaksinna urinarybiomarkersinacutepyelonephritis
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