Ability of Fourteen Chemical Agents Used in Dental Practice to Induce Chromosome Aberrations in Syrian Hamster Embryo Cells

Ability of Fourteen Chemical Agents Used in Dental Practice to Induce Chromosome Aberrations in Syrian Hamster Embryo Cells

To assess the genotoxicity of 14 chemical agents used in dental practice, the ability of these agents to induce chromosome aberrations was examined using Syrian hamster embryo (SHE) cells. Statistically significant increases in the frequencies of chromosome aberrations were induced in SHE cells trea...

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Main Authors: Hirohito Hikiba, Eiko Watanabe, J. Carl Barrett, Takeki Tsutsui
Format: Article
Language:English
Published: Elsevier 2005-01-01
Series:Journal of Pharmacological Sciences
Online Access:http://www.sciencedirect.com/science/article/pii/S1347861319323059
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spelling doaj-8fd894e2707747ad8a35183fb99128f52020-11-25T02:06:26ZengElsevierJournal of Pharmacological Sciences1347-86132005-01-01971146152Ability of Fourteen Chemical Agents Used in Dental Practice to Induce Chromosome Aberrations in Syrian Hamster Embryo CellsHirohito Hikiba0Eiko Watanabe1J. Carl Barrett2Takeki Tsutsui3Department of Pharmacology, The Nippon Dental University, School of Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, JapanDepartment of Pharmacology, The Nippon Dental University, School of Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, JapanLaboratory of Biosystems and Cancer, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USADepartment of Pharmacology, The Nippon Dental University, School of Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, Japan; Corresponding author. FAX: +81-3-3263-5452 E-mail: takeki@tokyo.ndu.ac.jpTo assess the genotoxicity of 14 chemical agents used in dental practice, the ability of these agents to induce chromosome aberrations was examined using Syrian hamster embryo (SHE) cells. Statistically significant increases in the frequencies of chromosome aberrations were induced in SHE cells treated with 7 of 10 chemical agents used as endodontic medicaments, that is, carbol camphor, m-cresol, eugenol, guaiacol, zinc oxide, hydrogen peroxide, and formaldehyde. The other 3 chemical agents, that is, thymol, glutaraldehyde, and iodoform, did not increase the levels of chromosome aberrations. Of the 4 chemical agents that are used as an antiseptic on the oral mucosa, chromosome aberrations were induced by iodine, but not by the other 3 antiseptics, benzalkonium chloride, benzethonium chloride, and chlorhexidine. Among the 6 chemical agents exhibiting a negative response in the assay, only thymol induced chromosome aberrations in the presence of exogenous metabolic activation. Our results indicate that chemical agents having a positive response in the present study are potentially genotoxic to mammalian cells and need to be studied further in detail. Keywords:: chromosome aberration, chemical agents used in dentistry, Syrian hamster embryo cellhttp://www.sciencedirect.com/science/article/pii/S1347861319323059
collection DOAJ
language English
format Article
sources DOAJ
author Hirohito Hikiba
Eiko Watanabe
J. Carl Barrett
Takeki Tsutsui
spellingShingle Hirohito Hikiba
Eiko Watanabe
J. Carl Barrett
Takeki Tsutsui
Ability of Fourteen Chemical Agents Used in Dental Practice to Induce Chromosome Aberrations in Syrian Hamster Embryo Cells
Journal of Pharmacological Sciences
author_facet Hirohito Hikiba
Eiko Watanabe
J. Carl Barrett
Takeki Tsutsui
author_sort Hirohito Hikiba
title Ability of Fourteen Chemical Agents Used in Dental Practice to Induce Chromosome Aberrations in Syrian Hamster Embryo Cells
title_short Ability of Fourteen Chemical Agents Used in Dental Practice to Induce Chromosome Aberrations in Syrian Hamster Embryo Cells
title_full Ability of Fourteen Chemical Agents Used in Dental Practice to Induce Chromosome Aberrations in Syrian Hamster Embryo Cells
title_fullStr Ability of Fourteen Chemical Agents Used in Dental Practice to Induce Chromosome Aberrations in Syrian Hamster Embryo Cells
title_full_unstemmed Ability of Fourteen Chemical Agents Used in Dental Practice to Induce Chromosome Aberrations in Syrian Hamster Embryo Cells
title_sort ability of fourteen chemical agents used in dental practice to induce chromosome aberrations in syrian hamster embryo cells
publisher Elsevier
series Journal of Pharmacological Sciences
issn 1347-8613
publishDate 2005-01-01
description To assess the genotoxicity of 14 chemical agents used in dental practice, the ability of these agents to induce chromosome aberrations was examined using Syrian hamster embryo (SHE) cells. Statistically significant increases in the frequencies of chromosome aberrations were induced in SHE cells treated with 7 of 10 chemical agents used as endodontic medicaments, that is, carbol camphor, m-cresol, eugenol, guaiacol, zinc oxide, hydrogen peroxide, and formaldehyde. The other 3 chemical agents, that is, thymol, glutaraldehyde, and iodoform, did not increase the levels of chromosome aberrations. Of the 4 chemical agents that are used as an antiseptic on the oral mucosa, chromosome aberrations were induced by iodine, but not by the other 3 antiseptics, benzalkonium chloride, benzethonium chloride, and chlorhexidine. Among the 6 chemical agents exhibiting a negative response in the assay, only thymol induced chromosome aberrations in the presence of exogenous metabolic activation. Our results indicate that chemical agents having a positive response in the present study are potentially genotoxic to mammalian cells and need to be studied further in detail. Keywords:: chromosome aberration, chemical agents used in dentistry, Syrian hamster embryo cell
url http://www.sciencedirect.com/science/article/pii/S1347861319323059
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