<it>ParaHox </it>gene expression in larval and postlarval development of the polychaete <it>Nereis virens </it>(Annelida, Lophotrochozoa)

• Main Authors: Andreeva Tatiana F, Cook Charles E, Kulakova Milana A
• Format: Article
• Language: English
• Published: BMC 2008-05-01
• Series: BMC Developmental Biology
• Online Access: http://www.biomedcentral.com/1471-213X/8/61

<p>Abstract</p> <p>Background</p> <p>Transcription factors that encode ANTP-class homeobox genes play crucial roles in determining the body plan organization and specification of different organs and tissues in bilaterian animals. The three-gene <it>ParaHox </it>family descends from an ancestral gene cluster that existed before the evolution of the Bilateria. All three <it>ParaHox </it>genes are reported from deuterostomes and lophotrochozoans, but not to date from any ecdysozoan taxa, and there is evidence that the <it>ParaHox </it>genes, like the related <it>Hox </it>genes, were ancestrally a single chromosomal cluster. However, unlike the <it>Hox </it>genes, there is as yet no strong evidence that the <it>ParaHox </it>genes are expressed in spatial and temporal order during embryogenesis.</p> <p>Results</p> <p>We isolated fragments of the three <it>Nereis virens ParaHox </it>genes, then used these as probes for whole-mount in situ hybridization in larval and postlarval worms. In <it>Nereis virens </it>the <it>ParaHox </it>genes participate in antero-posterior patterning of ectodermal and endodermal regions of the digestive tract and are expressed in some cells in the segment ganglia. The expression of these genes occurs in larval development in accordance with the position of these cells along the main body axis and in postlarval development in accordance with the position of cells in ganglia along the antero-posterior axis of each segment. In none of these tissues does expression of the three <it>ParaHox </it>genes follow the rule of temporal collinearity.</p> <p>Conclusion</p> <p>In <it>Nereis virens </it>the <it>ParaHox </it>genes are expressed during antero-posterior patterning of the digestive system (ectodermal foregut and hindgut, and endodermal midgut) of <it>Nereis virens</it>. These genes are also expressed during axial specification of ventral neuroectodermal cell domains, where the expression domains of each gene are re-iterated in each neuromere except for the first parapodial segment. These expression domains are probably predetermined and may be directed on the antero-posterior axis by the <it>Hox </it>genes, whose expression starts much earlier during embryogenesis. Our results support the hypothesis that the <it>ParaHox </it>genes are involved in antero-posterior patterning of the developing embryo, but they do not support the notion that these genes function only in the patterning of endodermal tissues.</p>