Heterozygous diploid structure of Amorphotheca resinae ZN1 contributes efficient biodetoxification on solid pretreated corn stover

Abstract Background Fast, complete, and ultimate removal of inhibitory compounds derived from lignocellulose pretreatment is the prerequisite for efficient production of cellulosic ethanol and biochemicals. Biodetoxification is the most promising method for inhibitor removal by its unique advantages...

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Main Authors:	Xia Yi, Qiuqiang Gao, Lei Zhang, Xia Wang, Yanqing He, Fengxian Hu, Jian Zhang, Gen Zou, Shihui Yang, Zhihua Zhou, Jie Bao
Format:	Article
Language:	English
Published:	BMC 2019-05-01
Series:	Biotechnology for Biofuels
Subjects:	Biodetoxification Amorphotheca resinae ZN1 Heterozygous diploid Gene pair Coordinate expression
Online Access:	http://link.springer.com/article/10.1186/s13068-019-1466-z

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spelling	doaj-8f85a409b0164a9aa41117441ca216a12020-11-25T03:14:18ZengBMCBiotechnology for Biofuels1754-68342019-05-0112111810.1186/s13068-019-1466-zHeterozygous diploid structure of Amorphotheca resinae ZN1 contributes efficient biodetoxification on solid pretreated corn stoverXia Yi0Qiuqiang Gao1Lei Zhang2Xia Wang3Yanqing He4Fengxian Hu5Jian Zhang6Gen Zou7Shihui Yang8Zhihua Zhou9Jie Bao10State Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyCAS Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyCAS Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesHubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei UniversityCAS Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyAbstract Background Fast, complete, and ultimate removal of inhibitory compounds derived from lignocellulose pretreatment is the prerequisite for efficient production of cellulosic ethanol and biochemicals. Biodetoxification is the most promising method for inhibitor removal by its unique advantages. The biodetoxification mechanisms of a unique diploid fungus responsible for highly efficient biodetoxification in solid-state culture was extensively investigated in the aspects of cellular structure, genome sequencing, transcriptome analysis, and practical biodetoxification. Results The inborn heterozygous diploid structure of A. resinae ZN1 uniquely contributed to the enhancement of inhibitor tolerance and conversion. The co-expression of gene pairs contributed to the enhancement of the degradation of lignocellulose-derived model inhibitors. The ultimate inhibitors degradation pathways and sugar conservation were elucidated by microbial degradation experimentation as well as the genomic and transcriptomic sequencing analysis. Conclusions The finding of the heterozygous diploid structure in A. resinae ZN1 on biodetoxification took the first insight into the global overview of biodetoxification mechanism of lignocellulose-derived inhibitors. This study provided a unique and practical biodetoxification biocatalyst of inhibitor compounds for lignocellulose biorefinery processing, as well as the synthetic biology tools on biodetoxification of biorefinery fermenting strains.http://link.springer.com/article/10.1186/s13068-019-1466-zBiodetoxificationAmorphotheca resinae ZN1Heterozygous diploidGene pairCoordinate expression
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Xia Yi Qiuqiang Gao Lei Zhang Xia Wang Yanqing He Fengxian Hu Jian Zhang Gen Zou Shihui Yang Zhihua Zhou Jie Bao
spellingShingle	Xia Yi Qiuqiang Gao Lei Zhang Xia Wang Yanqing He Fengxian Hu Jian Zhang Gen Zou Shihui Yang Zhihua Zhou Jie Bao Heterozygous diploid structure of Amorphotheca resinae ZN1 contributes efficient biodetoxification on solid pretreated corn stover Biotechnology for Biofuels Biodetoxification Amorphotheca resinae ZN1 Heterozygous diploid Gene pair Coordinate expression
author_facet	Xia Yi Qiuqiang Gao Lei Zhang Xia Wang Yanqing He Fengxian Hu Jian Zhang Gen Zou Shihui Yang Zhihua Zhou Jie Bao
author_sort	Xia Yi
title	Heterozygous diploid structure of Amorphotheca resinae ZN1 contributes efficient biodetoxification on solid pretreated corn stover
title_short	Heterozygous diploid structure of Amorphotheca resinae ZN1 contributes efficient biodetoxification on solid pretreated corn stover
title_full	Heterozygous diploid structure of Amorphotheca resinae ZN1 contributes efficient biodetoxification on solid pretreated corn stover
title_fullStr	Heterozygous diploid structure of Amorphotheca resinae ZN1 contributes efficient biodetoxification on solid pretreated corn stover
title_full_unstemmed	Heterozygous diploid structure of Amorphotheca resinae ZN1 contributes efficient biodetoxification on solid pretreated corn stover
title_sort	heterozygous diploid structure of amorphotheca resinae zn1 contributes efficient biodetoxification on solid pretreated corn stover
publisher	BMC
series	Biotechnology for Biofuels
issn	1754-6834
publishDate	2019-05-01
description	Abstract Background Fast, complete, and ultimate removal of inhibitory compounds derived from lignocellulose pretreatment is the prerequisite for efficient production of cellulosic ethanol and biochemicals. Biodetoxification is the most promising method for inhibitor removal by its unique advantages. The biodetoxification mechanisms of a unique diploid fungus responsible for highly efficient biodetoxification in solid-state culture was extensively investigated in the aspects of cellular structure, genome sequencing, transcriptome analysis, and practical biodetoxification. Results The inborn heterozygous diploid structure of A. resinae ZN1 uniquely contributed to the enhancement of inhibitor tolerance and conversion. The co-expression of gene pairs contributed to the enhancement of the degradation of lignocellulose-derived model inhibitors. The ultimate inhibitors degradation pathways and sugar conservation were elucidated by microbial degradation experimentation as well as the genomic and transcriptomic sequencing analysis. Conclusions The finding of the heterozygous diploid structure in A. resinae ZN1 on biodetoxification took the first insight into the global overview of biodetoxification mechanism of lignocellulose-derived inhibitors. This study provided a unique and practical biodetoxification biocatalyst of inhibitor compounds for lignocellulose biorefinery processing, as well as the synthetic biology tools on biodetoxification of biorefinery fermenting strains.
topic	Biodetoxification Amorphotheca resinae ZN1 Heterozygous diploid Gene pair Coordinate expression
url	http://link.springer.com/article/10.1186/s13068-019-1466-z
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Heterozygous diploid structure of Amorphotheca resinae ZN1 contributes efficient biodetoxification on solid pretreated corn stover

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