Induced pluripotent stem cells line (UNIPDi003-A) from a patient affected by EEC syndrome carrying the R279H mutation in TP63 gene

Induced pluripotent stem cells line (UNIPDi003-A) from a patient affected by EEC syndrome carrying the R279H mutation in TP63 gene

Oral mucosa epithelial stem cells from a patient affected by Ectrodactyly-Ectodermal dysplasia-Clefting (EEC) syndrome carrying the R279H mutation in the TP63 gene were reprogrammed into human induced pluripotent stem cells (hiPSCs) with episomal vectors. The generated UNIPDi003-A-hPSC line retained...

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Main Authors: Marta Trevisan, Enzo Di Iorio, Giulia Masi, Silvia Riccetti, Luisa Barzon, Gualtiero Alvisi, Luciana Caenazzo, Vanessa Barbaro, Giorgio Palù
Format: Article
Language:English
Published: Elsevier 2018-04-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506118300527
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spelling doaj-8f4ee27df52447488f19c9bbfe48686b2020-11-24T22:15:05ZengElsevierStem Cell Research1873-50612018-04-0128141144Induced pluripotent stem cells line (UNIPDi003-A) from a patient affected by EEC syndrome carrying the R279H mutation in TP63 geneMarta Trevisan0Enzo Di Iorio1Giulia Masi2Silvia Riccetti3Luisa Barzon4Gualtiero Alvisi5Luciana Caenazzo6Vanessa Barbaro7Giorgio Palù8Department of Molecular Medicine, University of Padova, 35121 Padua, Italy; Corresponding author.Department of Molecular Medicine, University of Padova, 35121 Padua, ItalyDepartment of Molecular Medicine, University of Padova, 35121 Padua, ItalyDepartment of Molecular Medicine, University of Padova, 35121 Padua, ItalyDepartment of Molecular Medicine, University of Padova, 35121 Padua, ItalyDepartment of Molecular Medicine, University of Padova, 35121 Padua, ItalyDepartment of Molecular Medicine, University of Padova, 35121 Padua, ItalyFondazione Banca degli Occhi del Veneto, 30174 Venice, ItalyDepartment of Molecular Medicine, University of Padova, 35121 Padua, ItalyOral mucosa epithelial stem cells from a patient affected by Ectrodactyly-Ectodermal dysplasia-Clefting (EEC) syndrome carrying the R279H mutation in the TP63 gene were reprogrammed into human induced pluripotent stem cells (hiPSCs) with episomal vectors. The generated UNIPDi003-A-hPSC line retained the mutation of the parental cells and showed a normal karyotype upon long term culture. Analysis of residual transgenes expression showed that the episomal vectors were eliminated from the cell line. UNIPDi003-A-hiPSCs expressed the undifferentiated state marker alkaline phosphatase along with a panel of pluripotency markers, and formed embryoid bodies capable of expressing markers belonging to all the three germ layers.http://www.sciencedirect.com/science/article/pii/S1873506118300527
collection DOAJ
language English
format Article
sources DOAJ
author Marta Trevisan
Enzo Di Iorio
Giulia Masi
Silvia Riccetti
Luisa Barzon
Gualtiero Alvisi
Luciana Caenazzo
Vanessa Barbaro
Giorgio Palù
spellingShingle Marta Trevisan
Enzo Di Iorio
Giulia Masi
Silvia Riccetti
Luisa Barzon
Gualtiero Alvisi
Luciana Caenazzo
Vanessa Barbaro
Giorgio Palù
Induced pluripotent stem cells line (UNIPDi003-A) from a patient affected by EEC syndrome carrying the R279H mutation in TP63 gene
Stem Cell Research
author_facet Marta Trevisan
Enzo Di Iorio
Giulia Masi
Silvia Riccetti
Luisa Barzon
Gualtiero Alvisi
Luciana Caenazzo
Vanessa Barbaro
Giorgio Palù
author_sort Marta Trevisan
title Induced pluripotent stem cells line (UNIPDi003-A) from a patient affected by EEC syndrome carrying the R279H mutation in TP63 gene
title_short Induced pluripotent stem cells line (UNIPDi003-A) from a patient affected by EEC syndrome carrying the R279H mutation in TP63 gene
title_full Induced pluripotent stem cells line (UNIPDi003-A) from a patient affected by EEC syndrome carrying the R279H mutation in TP63 gene
title_fullStr Induced pluripotent stem cells line (UNIPDi003-A) from a patient affected by EEC syndrome carrying the R279H mutation in TP63 gene
title_full_unstemmed Induced pluripotent stem cells line (UNIPDi003-A) from a patient affected by EEC syndrome carrying the R279H mutation in TP63 gene
title_sort induced pluripotent stem cells line (unipdi003-a) from a patient affected by eec syndrome carrying the r279h mutation in tp63 gene
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2018-04-01
description Oral mucosa epithelial stem cells from a patient affected by Ectrodactyly-Ectodermal dysplasia-Clefting (EEC) syndrome carrying the R279H mutation in the TP63 gene were reprogrammed into human induced pluripotent stem cells (hiPSCs) with episomal vectors. The generated UNIPDi003-A-hPSC line retained the mutation of the parental cells and showed a normal karyotype upon long term culture. Analysis of residual transgenes expression showed that the episomal vectors were eliminated from the cell line. UNIPDi003-A-hiPSCs expressed the undifferentiated state marker alkaline phosphatase along with a panel of pluripotency markers, and formed embryoid bodies capable of expressing markers belonging to all the three germ layers.
url http://www.sciencedirect.com/science/article/pii/S1873506118300527
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