PFA fixation enables artifact-free super-resolution imaging of the actin cytoskeleton and associated proteins

PFA fixation enables artifact-free super-resolution imaging of the actin cytoskeleton and associated proteins

Super-resolution microscopy (SRM) allows precise localization of proteins in cellular organelles and structures, including the actin cytoskeleton. Yet sample preparation protocols for SRM are rather anecdotal and still being optimized. Thus, SRM-based imaging of the actin cytoskeleton and associated...

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Bibliographic Details
Main Authors: Daniela Leyton-Puig, Katarzyna M. Kedziora, Tadamoto Isogai, Bram van den Broek, Kees Jalink, Metello Innocenti
Format: Article
Language:English
Published: The Company of Biologists 2016-07-01
Series:Biology Open
Subjects:
Super-resolution microscopy (SRM)
Protein localization
dSTORM
Fixation
Actin cytoskeleton
Online Access:http://bio.biologists.org/content/5/7/1001

Internet

http://bio.biologists.org/content/5/7/1001

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