N-trans-Feruloyloctopamine Wakes Up BBC3, DDIT3, CDKN1A, and NOXA Signals to Accelerate HCC Cell Apoptosis

N-trans-Feruloyloctopamine (FO), a natural compound, was reported in our previous study to inhibit a tumor cell malignant phenotype by AKT- and EMT-related signals and might be used as a promising drug for HCC treatment. However, the specific targets and detailed mechanisms still need to be clarifie...

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Main Authors: Bin Ma, Jing Li, Wen-Ke Yang, Mei-Gui Zhang, Xiao-Dong Xie, Zhong-Tian Bai
Format: Article
Language:English
Published: Hindawi Limited 2021-01-01
Series:Analytical Cellular Pathology
Online Access:http://dx.doi.org/10.1155/2021/1560307
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spelling doaj-8f27602200d34dc9add51de5dba5be2a2021-07-02T21:43:22ZengHindawi LimitedAnalytical Cellular Pathology2210-71852021-01-01202110.1155/2021/1560307N-trans-Feruloyloctopamine Wakes Up BBC3, DDIT3, CDKN1A, and NOXA Signals to Accelerate HCC Cell ApoptosisBin Ma0Jing Li1Wen-Ke Yang2Mei-Gui Zhang3Xiao-Dong Xie4Zhong-Tian Bai5Key Laboratory of Preclinical Study for New Drug of Gansu ProvinceGeneral Surgery DepartmentKey Laboratory of Preclinical Study for New Drug of Gansu ProvinceGeneral Surgery DepartmentKey Laboratory of Preclinical Study for New Drug of Gansu ProvinceGeneral Surgery DepartmentN-trans-Feruloyloctopamine (FO), a natural compound, was reported in our previous study to inhibit a tumor cell malignant phenotype by AKT- and EMT-related signals and might be used as a promising drug for HCC treatment. However, the specific targets and detailed mechanisms still need to be clarified. Screening with RNA-Seq in Huh7 cells treated with FO revealed that 317 genes were modulated, of which 188 genes were upregulated and 129 genes were downregulated. Real-time cell analyzer and flow cytometry data reveal that tumor cell proliferation and apoptosis were impacted by FO. DAVID bioinformatic data showed that most of the biological process GO terms are related to proliferation and apoptosis. KEGG enrichment analysis showed that FO mainly regulates PI3K-AKT- and apoptosis-related signals, in which BBC3, DDIT3, NOXA, and CDKN1A on the surface serve as the novel targets of FO inducing HCC cell apoptosis. The result implied that FO might exacerbate HCC cell apoptosis by regulating BBC3, DDIT3, CDKN1A, and NOXA signals. The obstacle effect of FO can provide new targets and new credibility for the treatment of liver cancer.http://dx.doi.org/10.1155/2021/1560307
collection DOAJ
language English
format Article
sources DOAJ
author Bin Ma
Jing Li
Wen-Ke Yang
Mei-Gui Zhang
Xiao-Dong Xie
Zhong-Tian Bai
spellingShingle Bin Ma
Jing Li
Wen-Ke Yang
Mei-Gui Zhang
Xiao-Dong Xie
Zhong-Tian Bai
N-trans-Feruloyloctopamine Wakes Up BBC3, DDIT3, CDKN1A, and NOXA Signals to Accelerate HCC Cell Apoptosis
Analytical Cellular Pathology
author_facet Bin Ma
Jing Li
Wen-Ke Yang
Mei-Gui Zhang
Xiao-Dong Xie
Zhong-Tian Bai
author_sort Bin Ma
title N-trans-Feruloyloctopamine Wakes Up BBC3, DDIT3, CDKN1A, and NOXA Signals to Accelerate HCC Cell Apoptosis
title_short N-trans-Feruloyloctopamine Wakes Up BBC3, DDIT3, CDKN1A, and NOXA Signals to Accelerate HCC Cell Apoptosis
title_full N-trans-Feruloyloctopamine Wakes Up BBC3, DDIT3, CDKN1A, and NOXA Signals to Accelerate HCC Cell Apoptosis
title_fullStr N-trans-Feruloyloctopamine Wakes Up BBC3, DDIT3, CDKN1A, and NOXA Signals to Accelerate HCC Cell Apoptosis
title_full_unstemmed N-trans-Feruloyloctopamine Wakes Up BBC3, DDIT3, CDKN1A, and NOXA Signals to Accelerate HCC Cell Apoptosis
title_sort n-trans-feruloyloctopamine wakes up bbc3, ddit3, cdkn1a, and noxa signals to accelerate hcc cell apoptosis
publisher Hindawi Limited
series Analytical Cellular Pathology
issn 2210-7185
publishDate 2021-01-01
description N-trans-Feruloyloctopamine (FO), a natural compound, was reported in our previous study to inhibit a tumor cell malignant phenotype by AKT- and EMT-related signals and might be used as a promising drug for HCC treatment. However, the specific targets and detailed mechanisms still need to be clarified. Screening with RNA-Seq in Huh7 cells treated with FO revealed that 317 genes were modulated, of which 188 genes were upregulated and 129 genes were downregulated. Real-time cell analyzer and flow cytometry data reveal that tumor cell proliferation and apoptosis were impacted by FO. DAVID bioinformatic data showed that most of the biological process GO terms are related to proliferation and apoptosis. KEGG enrichment analysis showed that FO mainly regulates PI3K-AKT- and apoptosis-related signals, in which BBC3, DDIT3, NOXA, and CDKN1A on the surface serve as the novel targets of FO inducing HCC cell apoptosis. The result implied that FO might exacerbate HCC cell apoptosis by regulating BBC3, DDIT3, CDKN1A, and NOXA signals. The obstacle effect of FO can provide new targets and new credibility for the treatment of liver cancer.
url http://dx.doi.org/10.1155/2021/1560307
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