A preliminary study on treatment of human breast cancer xenografts with a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (9C4)
Triple-negative breast cancer often has devastating outcomes and treatment options remain limited. Therefore, different treatment combinations are worthy of testing. The efficacy of a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (EpCAM) (9C4) to treat breast c...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2019-01-01
|
Series: | World Journal of Nuclear Medicine |
Subjects: | |
Online Access: | http://www.wjnm.org/article.asp?issn=1450-1147;year=2019;volume=18;issue=1;spage=18;epage=24;aulast=Ali |
id |
doaj-8f1ccdd1bdab41688b5b2e557b094ad5 |
---|---|
record_format |
Article |
spelling |
doaj-8f1ccdd1bdab41688b5b2e557b094ad52020-11-25T00:50:35ZengWolters Kluwer Medknow PublicationsWorld Journal of Nuclear Medicine1450-11472019-01-01181182410.4103/wjnm.WJNM_9_18A preliminary study on treatment of human breast cancer xenografts with a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (9C4)Naiim S AliJohn M AkuduguRoger W HowellTriple-negative breast cancer often has devastating outcomes and treatment options remain limited. Therefore, different treatment combinations are worthy of testing. The efficacy of a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (EpCAM) (9C4) to treat breast cancer was tested. Efficacy was tested with an MDA-MB-231 human breast cancer xenograft model. Anti-EpCAM (9C4) was demonstrated to bind to MDA-MB-231 human adenocarcinoma cells in vitro. Subsequently, mice-bearing MDA-MB-231× enografts were treated with either 131I-anti-EpCAM (9C4), unlabeled anti-EpCAM (9C4), paclitaxel, doxorubicin, or a cocktail of all of the agents. Tumor volume was measured for up to 70-day postinjection. Exponential regression was performed on tumor growth curves for each of the therapy groups. Statistical comparison of the growth constants λ of the regression models for each of the treatment groups with that of the cold antibody and control groups was done using extra sum-of-square F-tests. Biexponential clearance of 131I-anti-EpCAM (9C4) was observed with biological clearance half-times of 1.14 and 17.6 days for the first and second components, respectively. The mean growth rate of the tumors in animals treated with a cocktail of all of the agents was slower than in those treated with unlabeled anti-EpCAM (9C4) (P = 0.022). These preliminary data suggest that a cocktail of 131I-anti-EpCAM (9C4), paclitaxel, and doxorubicin may be suitable for treating breast cancers with high expression of EpCAM.http://www.wjnm.org/article.asp?issn=1450-1147;year=2019;volume=18;issue=1;spage=18;epage=24;aulast=AliChemotherapydoxorubicinepithelial cell adhesion moleculeiodine-131monoclonal antibodypaclitaxelradioimmunotherapy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Naiim S Ali John M Akudugu Roger W Howell |
spellingShingle |
Naiim S Ali John M Akudugu Roger W Howell A preliminary study on treatment of human breast cancer xenografts with a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (9C4) World Journal of Nuclear Medicine Chemotherapy doxorubicin epithelial cell adhesion molecule iodine-131 monoclonal antibody paclitaxel radioimmunotherapy |
author_facet |
Naiim S Ali John M Akudugu Roger W Howell |
author_sort |
Naiim S Ali |
title |
A preliminary study on treatment of human breast cancer xenografts with a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (9C4) |
title_short |
A preliminary study on treatment of human breast cancer xenografts with a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (9C4) |
title_full |
A preliminary study on treatment of human breast cancer xenografts with a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (9C4) |
title_fullStr |
A preliminary study on treatment of human breast cancer xenografts with a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (9C4) |
title_full_unstemmed |
A preliminary study on treatment of human breast cancer xenografts with a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (9C4) |
title_sort |
preliminary study on treatment of human breast cancer xenografts with a cocktail of paclitaxel, doxorubicin, and 131i-anti-epithelial cell adhesion molecule (9c4) |
publisher |
Wolters Kluwer Medknow Publications |
series |
World Journal of Nuclear Medicine |
issn |
1450-1147 |
publishDate |
2019-01-01 |
description |
Triple-negative breast cancer often has devastating outcomes and treatment options remain limited. Therefore, different treatment combinations are worthy of testing. The efficacy of a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (EpCAM) (9C4) to treat breast cancer was tested. Efficacy was tested with an MDA-MB-231 human breast cancer xenograft model. Anti-EpCAM (9C4) was demonstrated to bind to MDA-MB-231 human adenocarcinoma cells in vitro. Subsequently, mice-bearing MDA-MB-231× enografts were treated with either 131I-anti-EpCAM (9C4), unlabeled anti-EpCAM (9C4), paclitaxel, doxorubicin, or a cocktail of all of the agents. Tumor volume was measured for up to 70-day postinjection. Exponential regression was performed on tumor growth curves for each of the therapy groups. Statistical comparison of the growth constants λ of the regression models for each of the treatment groups with that of the cold antibody and control groups was done using extra sum-of-square F-tests. Biexponential clearance of 131I-anti-EpCAM (9C4) was observed with biological clearance half-times of 1.14 and 17.6 days for the first and second components, respectively. The mean growth rate of the tumors in animals treated with a cocktail of all of the agents was slower than in those treated with unlabeled anti-EpCAM (9C4) (P = 0.022). These preliminary data suggest that a cocktail of 131I-anti-EpCAM (9C4), paclitaxel, and doxorubicin may be suitable for treating breast cancers with high expression of EpCAM. |
topic |
Chemotherapy doxorubicin epithelial cell adhesion molecule iodine-131 monoclonal antibody paclitaxel radioimmunotherapy |
url |
http://www.wjnm.org/article.asp?issn=1450-1147;year=2019;volume=18;issue=1;spage=18;epage=24;aulast=Ali |
work_keys_str_mv |
AT naiimsali apreliminarystudyontreatmentofhumanbreastcancerxenograftswithacocktailofpaclitaxeldoxorubicinand131iantiepithelialcelladhesionmolecule9c4 AT johnmakudugu apreliminarystudyontreatmentofhumanbreastcancerxenograftswithacocktailofpaclitaxeldoxorubicinand131iantiepithelialcelladhesionmolecule9c4 AT rogerwhowell apreliminarystudyontreatmentofhumanbreastcancerxenograftswithacocktailofpaclitaxeldoxorubicinand131iantiepithelialcelladhesionmolecule9c4 AT naiimsali preliminarystudyontreatmentofhumanbreastcancerxenograftswithacocktailofpaclitaxeldoxorubicinand131iantiepithelialcelladhesionmolecule9c4 AT johnmakudugu preliminarystudyontreatmentofhumanbreastcancerxenograftswithacocktailofpaclitaxeldoxorubicinand131iantiepithelialcelladhesionmolecule9c4 AT rogerwhowell preliminarystudyontreatmentofhumanbreastcancerxenograftswithacocktailofpaclitaxeldoxorubicinand131iantiepithelialcelladhesionmolecule9c4 |
_version_ |
1725247716158603264 |