A preliminary study on treatment of human breast cancer xenografts with a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (9C4)

Triple-negative breast cancer often has devastating outcomes and treatment options remain limited. Therefore, different treatment combinations are worthy of testing. The efficacy of a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (EpCAM) (9C4) to treat breast c...

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Main Authors: Naiim S Ali, John M Akudugu, Roger W Howell
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2019-01-01
Series:World Journal of Nuclear Medicine
Subjects:
Online Access:http://www.wjnm.org/article.asp?issn=1450-1147;year=2019;volume=18;issue=1;spage=18;epage=24;aulast=Ali
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spelling doaj-8f1ccdd1bdab41688b5b2e557b094ad52020-11-25T00:50:35ZengWolters Kluwer Medknow PublicationsWorld Journal of Nuclear Medicine1450-11472019-01-01181182410.4103/wjnm.WJNM_9_18A preliminary study on treatment of human breast cancer xenografts with a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (9C4)Naiim S AliJohn M AkuduguRoger W HowellTriple-negative breast cancer often has devastating outcomes and treatment options remain limited. Therefore, different treatment combinations are worthy of testing. The efficacy of a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (EpCAM) (9C4) to treat breast cancer was tested. Efficacy was tested with an MDA-MB-231 human breast cancer xenograft model. Anti-EpCAM (9C4) was demonstrated to bind to MDA-MB-231 human adenocarcinoma cells in vitro. Subsequently, mice-bearing MDA-MB-231× enografts were treated with either 131I-anti-EpCAM (9C4), unlabeled anti-EpCAM (9C4), paclitaxel, doxorubicin, or a cocktail of all of the agents. Tumor volume was measured for up to 70-day postinjection. Exponential regression was performed on tumor growth curves for each of the therapy groups. Statistical comparison of the growth constants λ of the regression models for each of the treatment groups with that of the cold antibody and control groups was done using extra sum-of-square F-tests. Biexponential clearance of 131I-anti-EpCAM (9C4) was observed with biological clearance half-times of 1.14 and 17.6 days for the first and second components, respectively. The mean growth rate of the tumors in animals treated with a cocktail of all of the agents was slower than in those treated with unlabeled anti-EpCAM (9C4) (P = 0.022). These preliminary data suggest that a cocktail of 131I-anti-EpCAM (9C4), paclitaxel, and doxorubicin may be suitable for treating breast cancers with high expression of EpCAM.http://www.wjnm.org/article.asp?issn=1450-1147;year=2019;volume=18;issue=1;spage=18;epage=24;aulast=AliChemotherapydoxorubicinepithelial cell adhesion moleculeiodine-131monoclonal antibodypaclitaxelradioimmunotherapy
collection DOAJ
language English
format Article
sources DOAJ
author Naiim S Ali
John M Akudugu
Roger W Howell
spellingShingle Naiim S Ali
John M Akudugu
Roger W Howell
A preliminary study on treatment of human breast cancer xenografts with a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (9C4)
World Journal of Nuclear Medicine
Chemotherapy
doxorubicin
epithelial cell adhesion molecule
iodine-131
monoclonal antibody
paclitaxel
radioimmunotherapy
author_facet Naiim S Ali
John M Akudugu
Roger W Howell
author_sort Naiim S Ali
title A preliminary study on treatment of human breast cancer xenografts with a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (9C4)
title_short A preliminary study on treatment of human breast cancer xenografts with a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (9C4)
title_full A preliminary study on treatment of human breast cancer xenografts with a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (9C4)
title_fullStr A preliminary study on treatment of human breast cancer xenografts with a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (9C4)
title_full_unstemmed A preliminary study on treatment of human breast cancer xenografts with a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (9C4)
title_sort preliminary study on treatment of human breast cancer xenografts with a cocktail of paclitaxel, doxorubicin, and 131i-anti-epithelial cell adhesion molecule (9c4)
publisher Wolters Kluwer Medknow Publications
series World Journal of Nuclear Medicine
issn 1450-1147
publishDate 2019-01-01
description Triple-negative breast cancer often has devastating outcomes and treatment options remain limited. Therefore, different treatment combinations are worthy of testing. The efficacy of a cocktail of paclitaxel, doxorubicin, and 131I-anti-epithelial cell adhesion molecule (EpCAM) (9C4) to treat breast cancer was tested. Efficacy was tested with an MDA-MB-231 human breast cancer xenograft model. Anti-EpCAM (9C4) was demonstrated to bind to MDA-MB-231 human adenocarcinoma cells in vitro. Subsequently, mice-bearing MDA-MB-231× enografts were treated with either 131I-anti-EpCAM (9C4), unlabeled anti-EpCAM (9C4), paclitaxel, doxorubicin, or a cocktail of all of the agents. Tumor volume was measured for up to 70-day postinjection. Exponential regression was performed on tumor growth curves for each of the therapy groups. Statistical comparison of the growth constants λ of the regression models for each of the treatment groups with that of the cold antibody and control groups was done using extra sum-of-square F-tests. Biexponential clearance of 131I-anti-EpCAM (9C4) was observed with biological clearance half-times of 1.14 and 17.6 days for the first and second components, respectively. The mean growth rate of the tumors in animals treated with a cocktail of all of the agents was slower than in those treated with unlabeled anti-EpCAM (9C4) (P = 0.022). These preliminary data suggest that a cocktail of 131I-anti-EpCAM (9C4), paclitaxel, and doxorubicin may be suitable for treating breast cancers with high expression of EpCAM.
topic Chemotherapy
doxorubicin
epithelial cell adhesion molecule
iodine-131
monoclonal antibody
paclitaxel
radioimmunotherapy
url http://www.wjnm.org/article.asp?issn=1450-1147;year=2019;volume=18;issue=1;spage=18;epage=24;aulast=Ali
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