| Summary: | The authors selected the parameters for obtaining root cultures in vitro Medicago sativa. The number of cell culture lines studied is 15. The duration of the growth cycle is less than 50 days. The number of processed explants is 75. The growth characteristics of root cultures of in vitro purple alfalfa (Medicago sativa) were studied. The growth index for the dry biomass of the in vitro root culture of alfalfa was 31. A qualitative and quantitative analysis of the composition of BAS in the biomass of the obtained root cultures of in vitro alfalfa (Medicago sativa) was carried out. Unique biologically active substances (not previously described for alfalfa) were identified.): apigenin, naringenin, myricetin, chrysoeriol, coumestrol, and scopoletin. Rational parameters of isolation of individual BAS from the extract of root cultures of in vitro alfalfa (Medicago sativa) were selected. The degree of extraction of individual BAS is 80%. Methods of purification of individual BAS Medicago sativa have been developed. The degree of purification of individual BAS is 95%.
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