Studying nucleic envelope and plasma membrane mechanics of eukaryotic cells using confocal reflectance interferometric microscopy

Studying nucleic envelope and plasma membrane mechanics of eukaryotic cells using confocal reflectance interferometric microscopy

Biomechanical studies of eukaryotic cells have been limited due to low sensitivity and axial resolution in interferometric imaging. Here, the authors present depth-resolved confocal reflectance interferometric microscopy with high sensitivity and temporal resolution, which enables quantification of...

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Main Authors: Vijay Raj Singh, Yi An Yang, Hanry Yu, Roger D. Kamm, Zahid Yaqoob, Peter T. C. So
Format: Article
Language:English
Published: Nature Publishing Group 2019-08-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-11645-4
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spelling doaj-8ea1f37c4e17420a9c7f4bd577bf0c0e2021-05-11T12:06:50ZengNature Publishing GroupNature Communications2041-17232019-08-011011810.1038/s41467-019-11645-4Studying nucleic envelope and plasma membrane mechanics of eukaryotic cells using confocal reflectance interferometric microscopyVijay Raj Singh0Yi An Yang1Hanry Yu2Roger D. Kamm3Zahid Yaqoob4Peter T. C. So5Laser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of TechnologyMechanobiology Institute (MBI), National University of SingaporeSingapore-MIT Alliance for Research and TechnologySingapore-MIT Alliance for Research and TechnologyLaser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of TechnologyLaser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of TechnologyBiomechanical studies of eukaryotic cells have been limited due to low sensitivity and axial resolution in interferometric imaging. Here, the authors present depth-resolved confocal reflectance interferometric microscopy with high sensitivity and temporal resolution, which enables quantification of nucleic envelope and plasma membrane fluctuations.https://doi.org/10.1038/s41467-019-11645-4
collection DOAJ
language English
format Article
sources DOAJ
author Vijay Raj Singh
Yi An Yang
Hanry Yu
Roger D. Kamm
Zahid Yaqoob
Peter T. C. So
spellingShingle Vijay Raj Singh
Yi An Yang
Hanry Yu
Roger D. Kamm
Zahid Yaqoob
Peter T. C. So
Studying nucleic envelope and plasma membrane mechanics of eukaryotic cells using confocal reflectance interferometric microscopy
Nature Communications
author_facet Vijay Raj Singh
Yi An Yang
Hanry Yu
Roger D. Kamm
Zahid Yaqoob
Peter T. C. So
author_sort Vijay Raj Singh
title Studying nucleic envelope and plasma membrane mechanics of eukaryotic cells using confocal reflectance interferometric microscopy
title_short Studying nucleic envelope and plasma membrane mechanics of eukaryotic cells using confocal reflectance interferometric microscopy
title_full Studying nucleic envelope and plasma membrane mechanics of eukaryotic cells using confocal reflectance interferometric microscopy
title_fullStr Studying nucleic envelope and plasma membrane mechanics of eukaryotic cells using confocal reflectance interferometric microscopy
title_full_unstemmed Studying nucleic envelope and plasma membrane mechanics of eukaryotic cells using confocal reflectance interferometric microscopy
title_sort studying nucleic envelope and plasma membrane mechanics of eukaryotic cells using confocal reflectance interferometric microscopy
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2019-08-01
description Biomechanical studies of eukaryotic cells have been limited due to low sensitivity and axial resolution in interferometric imaging. Here, the authors present depth-resolved confocal reflectance interferometric microscopy with high sensitivity and temporal resolution, which enables quantification of nucleic envelope and plasma membrane fluctuations.
url https://doi.org/10.1038/s41467-019-11645-4
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AT yianyang studyingnucleicenvelopeandplasmamembranemechanicsofeukaryoticcellsusingconfocalreflectanceinterferometricmicroscopy
AT hanryyu studyingnucleicenvelopeandplasmamembranemechanicsofeukaryoticcellsusingconfocalreflectanceinterferometricmicroscopy
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