Kinetic studies on clinical and immunological modulations by intramuscular injection of LPS in laying hens

• Main Authors: Wendy Liermann, Jana Frahm, Ingrid Halle, Susanne Bühler, Jeannette Kluess, Liane Hüther, Sven Dänicke
• Format: Article
• Language: English
• Published: SAGE Publishing 2019-04-01
• Series: Innate Immunity
• Online Access: https://doi.org/10.1177/1753425919835296
• ISSN: 1753-4259; 1753-4267

The present study investigated clinical and immunological modulations due to intramuscular injection of Escherichia coli LPS in 49-wk-old laying hens over 48 h post injection (p.i.). LPS induced characteristic sickness behavior but no significant body temperature alterations ( P  > 0.05). During experimental period decreases in blood albumin, calcium, phosphorus and tryptophan concentrations, hyperglycemia, increased plasma nitrite concentrations, leucopenia, decreased thrombocyte counts, lymphopenia, heterophilia and an increased heterophilic granulocyte/lymphocyte (H/L) ratio were observed after LPS administration. Time-dependent effects were shown on T and B cell subsets in caecal tonsils (CT) and on splenic CD3 + /CD4 + /CD8 + proportions, on IL-1β and -10 and inducible NO synthase mRNA expression in peripheral blood lymphocytes (PBL), liver, spleen and CT, and on the mRNA expression of the TLR4 in PBL, liver and spleen p.i. ( P  < 0.05). The main responding period of mentioned alterations due to LPS appears to include the period from 2 until 8 h p.i. According to the H/L ratio, the most stressful phase was 5 h p.i. T and B cell subsets in CT, the IL-1β and TLR4 mRNA expression in liver and plasma nitrite concentrations seemed to be affected for a longer period.