Optimizing bacterial DNA extraction in urine.

Urine is an acceptable, non-invasive sample for investigating the human urogenital microbiota and for the diagnosis of sexually transmitted infections. However, low quantities of bacterial DNA and PCR inhibitors in urine may prevent efficient PCR amplification for molecular detection of bacteria. Fu...

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Bibliographic Details
Main Authors: Matthew M Munch, Laura C Chambers, Lisa E Manhart, Dan Domogala, Anthony Lopez, David N Fredricks, Sujatha Srinivasan
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0222962

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