IRS1/PI3K/AKT pathway signal involved in the regulation of glycolipid metabolic abnormalities by Mulberry (Morus alba L.) leaf extracts in 3T3-L1 adipocytes

Abstract Background Mulberry (Morus alba L.) leaf tea benefits the control of diabetes in Asian nations. This study was aim to investigate if the flavonoids, which extracts from mulberry leaves, could regulate the metabolism of glycolipid, and to investigate if flavonoids could regulate IRS1/PI3K/AK...

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Main Authors: Qinghai Meng, Xu Qi, Ying Chao, Qi Chen, Peng Cheng, Xichao Yu, Meiyu Kuai, Jingzhen Wu, Wenwen Li, Qichun Zhang, Yu Li, Huimin Bian
Format: Article
Language:English
Published: BMC 2020-01-01
Series:Chinese Medicine
Subjects:
Online Access:https://doi.org/10.1186/s13020-019-0281-6
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spelling doaj-8e216ab03ef4496398d438cfa7e67a372021-01-03T12:13:28ZengBMCChinese Medicine1749-85462020-01-0115111110.1186/s13020-019-0281-6IRS1/PI3K/AKT pathway signal involved in the regulation of glycolipid metabolic abnormalities by Mulberry (Morus alba L.) leaf extracts in 3T3-L1 adipocytesQinghai Meng0Xu Qi1Ying Chao2Qi Chen3Peng Cheng4Xichao Yu5Meiyu Kuai6Jingzhen Wu7Wenwen Li8Qichun Zhang9Yu Li10Huimin Bian11School of Pharmacy, Nanjing University of Chinese MedicineDepartment of Respiratory Medicine, The First Affiliated Hospital of Nanjing Medical UniversitySchool of Pharmacy, Nanjing University of Chinese MedicineSchool of Pharmacy, Nanjing University of Chinese MedicineSchool of Pharmacy, Nanjing University of Chinese MedicineSchool of Pharmacy, Nanjing University of Chinese MedicineSchool of Pharmacy, Nanjing University of Chinese MedicineSchool of Medicine and Life Sciences, Nanjing University of Chinese MedicineSchool of Medicine and Life Sciences, Nanjing University of Chinese MedicineSchool of Pharmacy, Nanjing University of Chinese MedicineSchool of Medicine and Life Sciences, Nanjing University of Chinese MedicineSchool of Pharmacy, Nanjing University of Chinese MedicineAbstract Background Mulberry (Morus alba L.) leaf tea benefits the control of diabetes in Asian nations. This study was aim to investigate if the flavonoids, which extracts from mulberry leaves, could regulate the metabolism of glycolipid, and to investigate if flavonoids could regulate IRS1/PI3K/AKT pathway signal to affect the expression of FAS and membrane transfer capacity GLUT4 in 3T3-L1 adipocytes. Results Results revealed that flavonoids decreased the levels of free fatty acid and increased the glucose consumption and the levels of adiponectin and leptin in a dose-dependent manner, and remarkably increased the protein expression levels of p-IRS1, p-PI3K, p-Akt, total GLUT4, and membrane GLUT4, and decreased the protein expression levels of PTEN and FAS in 3T3-L1 adipocytes IR model. On the other hand, wortmannin (2 nM), a selective and irreversible PI3K inhibitor, significantly decreased the glucose consumption and the adiponectin and leptin levels, and increased the free fatty acid level in flavonoids treated 3T3-L1 adipocytes IR model. Furthermore, wortmannin (2 nM) partly eliminated the activation of PI3K/AKT signaling, the suppression of FAS, and the up-regulated membrane transfer capacity of GLUT4 in flavonoids treated 3T3-L1 adipocytes IR model. Conclusion In conclusion, our results illustrated that mulberry leaf extracts flavonoids alleviated the glycolipid metabolic abnormalities in 3T3-L1 adipocytes IR model, and the effect was associated with the activation of IRS1/PI3K/AKT pathway, the suppression of FAS, and the up-regulation of membrane transfer capacity of GLUT4.https://doi.org/10.1186/s13020-019-0281-6Mulberry leafFlavonoidAdipocytesType 2 diabetesPI3K/AKT
collection DOAJ
language English
format Article
sources DOAJ
author Qinghai Meng
Xu Qi
Ying Chao
Qi Chen
Peng Cheng
Xichao Yu
Meiyu Kuai
Jingzhen Wu
Wenwen Li
Qichun Zhang
Yu Li
Huimin Bian
spellingShingle Qinghai Meng
Xu Qi
Ying Chao
Qi Chen
Peng Cheng
Xichao Yu
Meiyu Kuai
Jingzhen Wu
Wenwen Li
Qichun Zhang
Yu Li
Huimin Bian
IRS1/PI3K/AKT pathway signal involved in the regulation of glycolipid metabolic abnormalities by Mulberry (Morus alba L.) leaf extracts in 3T3-L1 adipocytes
Chinese Medicine
Mulberry leaf
Flavonoid
Adipocytes
Type 2 diabetes
PI3K/AKT
author_facet Qinghai Meng
Xu Qi
Ying Chao
Qi Chen
Peng Cheng
Xichao Yu
Meiyu Kuai
Jingzhen Wu
Wenwen Li
Qichun Zhang
Yu Li
Huimin Bian
author_sort Qinghai Meng
title IRS1/PI3K/AKT pathway signal involved in the regulation of glycolipid metabolic abnormalities by Mulberry (Morus alba L.) leaf extracts in 3T3-L1 adipocytes
title_short IRS1/PI3K/AKT pathway signal involved in the regulation of glycolipid metabolic abnormalities by Mulberry (Morus alba L.) leaf extracts in 3T3-L1 adipocytes
title_full IRS1/PI3K/AKT pathway signal involved in the regulation of glycolipid metabolic abnormalities by Mulberry (Morus alba L.) leaf extracts in 3T3-L1 adipocytes
title_fullStr IRS1/PI3K/AKT pathway signal involved in the regulation of glycolipid metabolic abnormalities by Mulberry (Morus alba L.) leaf extracts in 3T3-L1 adipocytes
title_full_unstemmed IRS1/PI3K/AKT pathway signal involved in the regulation of glycolipid metabolic abnormalities by Mulberry (Morus alba L.) leaf extracts in 3T3-L1 adipocytes
title_sort irs1/pi3k/akt pathway signal involved in the regulation of glycolipid metabolic abnormalities by mulberry (morus alba l.) leaf extracts in 3t3-l1 adipocytes
publisher BMC
series Chinese Medicine
issn 1749-8546
publishDate 2020-01-01
description Abstract Background Mulberry (Morus alba L.) leaf tea benefits the control of diabetes in Asian nations. This study was aim to investigate if the flavonoids, which extracts from mulberry leaves, could regulate the metabolism of glycolipid, and to investigate if flavonoids could regulate IRS1/PI3K/AKT pathway signal to affect the expression of FAS and membrane transfer capacity GLUT4 in 3T3-L1 adipocytes. Results Results revealed that flavonoids decreased the levels of free fatty acid and increased the glucose consumption and the levels of adiponectin and leptin in a dose-dependent manner, and remarkably increased the protein expression levels of p-IRS1, p-PI3K, p-Akt, total GLUT4, and membrane GLUT4, and decreased the protein expression levels of PTEN and FAS in 3T3-L1 adipocytes IR model. On the other hand, wortmannin (2 nM), a selective and irreversible PI3K inhibitor, significantly decreased the glucose consumption and the adiponectin and leptin levels, and increased the free fatty acid level in flavonoids treated 3T3-L1 adipocytes IR model. Furthermore, wortmannin (2 nM) partly eliminated the activation of PI3K/AKT signaling, the suppression of FAS, and the up-regulated membrane transfer capacity of GLUT4 in flavonoids treated 3T3-L1 adipocytes IR model. Conclusion In conclusion, our results illustrated that mulberry leaf extracts flavonoids alleviated the glycolipid metabolic abnormalities in 3T3-L1 adipocytes IR model, and the effect was associated with the activation of IRS1/PI3K/AKT pathway, the suppression of FAS, and the up-regulation of membrane transfer capacity of GLUT4.
topic Mulberry leaf
Flavonoid
Adipocytes
Type 2 diabetes
PI3K/AKT
url https://doi.org/10.1186/s13020-019-0281-6
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