Lymphoid Genes Expression in Mouse Embryonic Stem Cells with Differentiation on Feeder Free Condition

Lymphoid Genes Expression in Mouse Embryonic Stem Cells with Differentiation on Feeder Free Condition

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Embryonic stem cells (ESCs) as pluripotent stem cells have the potential to give rise to all three germ layer cells. Also, these cells have the self-renewing ability. ESCs could differentiate into most of cell lineages. Lymphocytes generated from hematopoietic stem cells. We designed a feeder free c...

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Bibliographic Details
Main Authors: Najmaldin Saki, Gholamreza Khamisipour, Saeid Abroun, Masoud Soleimani, Mina Soufizomorrod, Saeid Kaviani, Majid Mossahebimohammadi
Format: Article
Language:English
Published: Tehran University of Medical Sciences 2012-04-01
Series:International Journal of Hematology-Oncology and Stem Cell Research
Subjects:
Genes Expression
Mouse Embryonic Stem Cells
Differentiation
Feeder Free Condition
Online Access:http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/21109.pdf&manuscript_id=21109
Description
Summary:Embryonic stem cells (ESCs) as pluripotent stem cells have the potential to give rise to all three germ layer cells. Also, these cells have the self-renewing ability. ESCs could differentiate into most of cell lineages. Lymphocytes generated from hematopoietic stem cells. We designed a feeder free culture condition to generate lymphoid lineage cells from ESCs. We demonstrated that IL-7 plays critical role in differentiation of ESCs into lymphoid lineage and expression of specific cell markers such as CD3, CD19 and CD25 after 14 days of differentiation by RT-PCR.
ISSN:2008-3009
2008-2207

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