Anandamide uptake by synaptosomes from human, mouse and rat brain: inhibition by glutamine and glutamate

Anandamide uptake by synaptosomes from human, mouse and rat brain: inhibition by glutamine and glutamate

<p>Abstract</p> <p>Anandamide (<it>N</it>-arachidonoylethanolamine, AEA) belongs to an emerging class of endogenous lipids, called "endocannabinoids". A specific AEA membrane transporter (AMT) allows the import of this lipid and its degradation by the intracel...

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Main Authors: Finazzi-Agrò Alessandro, Bari Monica, Battista Natalia, Maccarrone Mauro
Format: Article
Language:English
Published: BMC 2002-09-01
Series:Lipids in Health and Disease
Online Access:http://www.lipidworld.com/content/1/1/1
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spelling doaj-8e07d4227aab42199a45f99dcff238fc2020-11-25T01:26:56ZengBMCLipids in Health and Disease1476-511X2002-09-0111110.1186/1476-511X-1-1Anandamide uptake by synaptosomes from human, mouse and rat brain: inhibition by glutamine and glutamateFinazzi-Agrò AlessandroBari MonicaBattista NataliaMaccarrone Mauro<p>Abstract</p> <p>Anandamide (<it>N</it>-arachidonoylethanolamine, AEA) belongs to an emerging class of endogenous lipids, called "endocannabinoids". A specific AEA membrane transporter (AMT) allows the import of this lipid and its degradation by the intracellular enzyme AEA hydrolase. Here, we show that synaptosomes from human, mouse and rat brain might be an ideal <it>ex vivo</it> system for the study of: i) the accumulation of AEA in brain, and ii) the pharmacological properties of AMT inhibitors. Using this <it>ex vivo</it> system, we demonstrate for the first time that glutamine and glutamate act as non-competitive inhibitors of AEA uptake by human, mouse and rat brain AMT.</p> http://www.lipidworld.com/content/1/1/1
collection DOAJ
language English
format Article
sources DOAJ
author Finazzi-Agrò Alessandro
Bari Monica
Battista Natalia
Maccarrone Mauro
spellingShingle Finazzi-Agrò Alessandro
Bari Monica
Battista Natalia
Maccarrone Mauro
Anandamide uptake by synaptosomes from human, mouse and rat brain: inhibition by glutamine and glutamate
Lipids in Health and Disease
author_facet Finazzi-Agrò Alessandro
Bari Monica
Battista Natalia
Maccarrone Mauro
author_sort Finazzi-Agrò Alessandro
title Anandamide uptake by synaptosomes from human, mouse and rat brain: inhibition by glutamine and glutamate
title_short Anandamide uptake by synaptosomes from human, mouse and rat brain: inhibition by glutamine and glutamate
title_full Anandamide uptake by synaptosomes from human, mouse and rat brain: inhibition by glutamine and glutamate
title_fullStr Anandamide uptake by synaptosomes from human, mouse and rat brain: inhibition by glutamine and glutamate
title_full_unstemmed Anandamide uptake by synaptosomes from human, mouse and rat brain: inhibition by glutamine and glutamate
title_sort anandamide uptake by synaptosomes from human, mouse and rat brain: inhibition by glutamine and glutamate
publisher BMC
series Lipids in Health and Disease
issn 1476-511X
publishDate 2002-09-01
description <p>Abstract</p> <p>Anandamide (<it>N</it>-arachidonoylethanolamine, AEA) belongs to an emerging class of endogenous lipids, called "endocannabinoids". A specific AEA membrane transporter (AMT) allows the import of this lipid and its degradation by the intracellular enzyme AEA hydrolase. Here, we show that synaptosomes from human, mouse and rat brain might be an ideal <it>ex vivo</it> system for the study of: i) the accumulation of AEA in brain, and ii) the pharmacological properties of AMT inhibitors. Using this <it>ex vivo</it> system, we demonstrate for the first time that glutamine and glutamate act as non-competitive inhibitors of AEA uptake by human, mouse and rat brain AMT.</p>
url http://www.lipidworld.com/content/1/1/1
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AT barimonica anandamideuptakebysynaptosomesfromhumanmouseandratbraininhibitionbyglutamineandglutamate
AT battistanatalia anandamideuptakebysynaptosomesfromhumanmouseandratbraininhibitionbyglutamineandglutamate
AT maccarronemauro anandamideuptakebysynaptosomesfromhumanmouseandratbraininhibitionbyglutamineandglutamate
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