Cadmium Concentration and Metallothionein Expression in Prostate Cancer and Benign Prostatic Hyperplasia of Humans

Cadmium (Cd) causes various genitourinary disorders and is a carcinogen for prostate cancer. Metallothionein (MT) is a protein that detoxifies heavy metals. We evaluated changes in Cd concentration and MT expression in human prostate cancer (CaP) and benign prostatic hyperplasia (BPH). Our goal was...

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Main Authors: Jane-Dar Lee, Su-Mei Wu, Lieng-Yi Lu, Ya-Tang Yang, Shaw-Yeu Jeng
Format: Article
Language:English
Published: Elsevier 2009-07-01
Series:Journal of the Formosan Medical Association
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0929664609603739
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spelling doaj-8e01373ed86543adb3fd48d0edec59b32020-11-24T23:41:31ZengElsevierJournal of the Formosan Medical Association0929-66462009-07-01108755455910.1016/S0929-6646(09)60373-9Cadmium Concentration and Metallothionein Expression in Prostate Cancer and Benign Prostatic Hyperplasia of HumansJane-Dar Lee0Su-Mei Wu1Lieng-Yi Lu2Ya-Tang Yang3Shaw-Yeu Jeng4Division of Urology, Department of Surgery, Taichung Armed Forces General Hospital, TaiwanDepartment of Aquatic Biosciences, National Chiayi University, Yuanshan Veterans Hospital, TaiwanDivision of Urology, Department of Surgery, Taichung Armed Forces General Hospital, TaiwanDepartment of Pathology, Taichung Armed Forces General Hospital, TaiwanDepartment of Surgery, Yuanshan Veterans Hospital, TaiwanCadmium (Cd) causes various genitourinary disorders and is a carcinogen for prostate cancer. Metallothionein (MT) is a protein that detoxifies heavy metals. We evaluated changes in Cd concentration and MT expression in human prostate cancer (CaP) and benign prostatic hyperplasia (BPH). Our goal was to clarify the relationship between Cd concentration and MT expression in prostatic diseases. Methods: The experimental group consisted of 18 patients who underwent radical prostatectomy for CaP. The control group consisted of 35 patients who underwent transurethral resection of the prostate for BPH. Tissue samples were acquired from the gross tumor site and from resected chips. We determined Cd concentration by atomic absorption, MT expression by immunoblotting, and immunohistochemical staining. The significance of between-group differences for these outcomes was analyzed using Student's t tests. Results: There was no statistically significant difference in Cd concentration between the CaP and BPH groups. Immunoblots from both groups revealed a single band. The relative intensity of the MT band was 0.58 ± 0.09 in the BPH group and 0.17 ± 0.03 in the CaP group. MT expression in patients with BPH was 3.4-fold higher than in those with CaP. Conclusion: MT may bind heavy metals and protect patients from CaP. Additional studies are needed to reveal the factors that influence the expression of MT in prostate epithelial cells, and to analyze the free and compound forms of Cd at the same time.http://www.sciencedirect.com/science/article/pii/S0929664609603739benign prostatic hyperplasiacadmiummetallothioneinprostate cancer
collection DOAJ
language English
format Article
sources DOAJ
author Jane-Dar Lee
Su-Mei Wu
Lieng-Yi Lu
Ya-Tang Yang
Shaw-Yeu Jeng
spellingShingle Jane-Dar Lee
Su-Mei Wu
Lieng-Yi Lu
Ya-Tang Yang
Shaw-Yeu Jeng
Cadmium Concentration and Metallothionein Expression in Prostate Cancer and Benign Prostatic Hyperplasia of Humans
Journal of the Formosan Medical Association
benign prostatic hyperplasia
cadmium
metallothionein
prostate cancer
author_facet Jane-Dar Lee
Su-Mei Wu
Lieng-Yi Lu
Ya-Tang Yang
Shaw-Yeu Jeng
author_sort Jane-Dar Lee
title Cadmium Concentration and Metallothionein Expression in Prostate Cancer and Benign Prostatic Hyperplasia of Humans
title_short Cadmium Concentration and Metallothionein Expression in Prostate Cancer and Benign Prostatic Hyperplasia of Humans
title_full Cadmium Concentration and Metallothionein Expression in Prostate Cancer and Benign Prostatic Hyperplasia of Humans
title_fullStr Cadmium Concentration and Metallothionein Expression in Prostate Cancer and Benign Prostatic Hyperplasia of Humans
title_full_unstemmed Cadmium Concentration and Metallothionein Expression in Prostate Cancer and Benign Prostatic Hyperplasia of Humans
title_sort cadmium concentration and metallothionein expression in prostate cancer and benign prostatic hyperplasia of humans
publisher Elsevier
series Journal of the Formosan Medical Association
issn 0929-6646
publishDate 2009-07-01
description Cadmium (Cd) causes various genitourinary disorders and is a carcinogen for prostate cancer. Metallothionein (MT) is a protein that detoxifies heavy metals. We evaluated changes in Cd concentration and MT expression in human prostate cancer (CaP) and benign prostatic hyperplasia (BPH). Our goal was to clarify the relationship between Cd concentration and MT expression in prostatic diseases. Methods: The experimental group consisted of 18 patients who underwent radical prostatectomy for CaP. The control group consisted of 35 patients who underwent transurethral resection of the prostate for BPH. Tissue samples were acquired from the gross tumor site and from resected chips. We determined Cd concentration by atomic absorption, MT expression by immunoblotting, and immunohistochemical staining. The significance of between-group differences for these outcomes was analyzed using Student's t tests. Results: There was no statistically significant difference in Cd concentration between the CaP and BPH groups. Immunoblots from both groups revealed a single band. The relative intensity of the MT band was 0.58 ± 0.09 in the BPH group and 0.17 ± 0.03 in the CaP group. MT expression in patients with BPH was 3.4-fold higher than in those with CaP. Conclusion: MT may bind heavy metals and protect patients from CaP. Additional studies are needed to reveal the factors that influence the expression of MT in prostate epithelial cells, and to analyze the free and compound forms of Cd at the same time.
topic benign prostatic hyperplasia
cadmium
metallothionein
prostate cancer
url http://www.sciencedirect.com/science/article/pii/S0929664609603739
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