Cadmium Concentration and Metallothionein Expression in Prostate Cancer and Benign Prostatic Hyperplasia of Humans
Cadmium (Cd) causes various genitourinary disorders and is a carcinogen for prostate cancer. Metallothionein (MT) is a protein that detoxifies heavy metals. We evaluated changes in Cd concentration and MT expression in human prostate cancer (CaP) and benign prostatic hyperplasia (BPH). Our goal was...
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2009-07-01
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doaj-8e01373ed86543adb3fd48d0edec59b32020-11-24T23:41:31ZengElsevierJournal of the Formosan Medical Association0929-66462009-07-01108755455910.1016/S0929-6646(09)60373-9Cadmium Concentration and Metallothionein Expression in Prostate Cancer and Benign Prostatic Hyperplasia of HumansJane-Dar Lee0Su-Mei Wu1Lieng-Yi Lu2Ya-Tang Yang3Shaw-Yeu Jeng4Division of Urology, Department of Surgery, Taichung Armed Forces General Hospital, TaiwanDepartment of Aquatic Biosciences, National Chiayi University, Yuanshan Veterans Hospital, TaiwanDivision of Urology, Department of Surgery, Taichung Armed Forces General Hospital, TaiwanDepartment of Pathology, Taichung Armed Forces General Hospital, TaiwanDepartment of Surgery, Yuanshan Veterans Hospital, TaiwanCadmium (Cd) causes various genitourinary disorders and is a carcinogen for prostate cancer. Metallothionein (MT) is a protein that detoxifies heavy metals. We evaluated changes in Cd concentration and MT expression in human prostate cancer (CaP) and benign prostatic hyperplasia (BPH). Our goal was to clarify the relationship between Cd concentration and MT expression in prostatic diseases. Methods: The experimental group consisted of 18 patients who underwent radical prostatectomy for CaP. The control group consisted of 35 patients who underwent transurethral resection of the prostate for BPH. Tissue samples were acquired from the gross tumor site and from resected chips. We determined Cd concentration by atomic absorption, MT expression by immunoblotting, and immunohistochemical staining. The significance of between-group differences for these outcomes was analyzed using Student's t tests. Results: There was no statistically significant difference in Cd concentration between the CaP and BPH groups. Immunoblots from both groups revealed a single band. The relative intensity of the MT band was 0.58 ± 0.09 in the BPH group and 0.17 ± 0.03 in the CaP group. MT expression in patients with BPH was 3.4-fold higher than in those with CaP. Conclusion: MT may bind heavy metals and protect patients from CaP. Additional studies are needed to reveal the factors that influence the expression of MT in prostate epithelial cells, and to analyze the free and compound forms of Cd at the same time.http://www.sciencedirect.com/science/article/pii/S0929664609603739benign prostatic hyperplasiacadmiummetallothioneinprostate cancer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jane-Dar Lee Su-Mei Wu Lieng-Yi Lu Ya-Tang Yang Shaw-Yeu Jeng |
spellingShingle |
Jane-Dar Lee Su-Mei Wu Lieng-Yi Lu Ya-Tang Yang Shaw-Yeu Jeng Cadmium Concentration and Metallothionein Expression in Prostate Cancer and Benign Prostatic Hyperplasia of Humans Journal of the Formosan Medical Association benign prostatic hyperplasia cadmium metallothionein prostate cancer |
author_facet |
Jane-Dar Lee Su-Mei Wu Lieng-Yi Lu Ya-Tang Yang Shaw-Yeu Jeng |
author_sort |
Jane-Dar Lee |
title |
Cadmium Concentration and Metallothionein Expression in Prostate Cancer and Benign Prostatic Hyperplasia of Humans |
title_short |
Cadmium Concentration and Metallothionein Expression in Prostate Cancer and Benign Prostatic Hyperplasia of Humans |
title_full |
Cadmium Concentration and Metallothionein Expression in Prostate Cancer and Benign Prostatic Hyperplasia of Humans |
title_fullStr |
Cadmium Concentration and Metallothionein Expression in Prostate Cancer and Benign Prostatic Hyperplasia of Humans |
title_full_unstemmed |
Cadmium Concentration and Metallothionein Expression in Prostate Cancer and Benign Prostatic Hyperplasia of Humans |
title_sort |
cadmium concentration and metallothionein expression in prostate cancer and benign prostatic hyperplasia of humans |
publisher |
Elsevier |
series |
Journal of the Formosan Medical Association |
issn |
0929-6646 |
publishDate |
2009-07-01 |
description |
Cadmium (Cd) causes various genitourinary disorders and is a carcinogen for prostate cancer. Metallothionein (MT) is a protein that detoxifies heavy metals. We evaluated changes in Cd concentration and MT expression in human prostate cancer (CaP) and benign prostatic hyperplasia (BPH). Our goal was to clarify the relationship between Cd concentration and MT expression in prostatic diseases.
Methods: The experimental group consisted of 18 patients who underwent radical prostatectomy for CaP. The control group consisted of 35 patients who underwent transurethral resection of the prostate for BPH. Tissue samples were acquired from the gross tumor site and from resected chips. We determined Cd concentration by atomic absorption, MT expression by immunoblotting, and immunohistochemical staining. The significance of between-group differences for these outcomes was analyzed using Student's t tests.
Results: There was no statistically significant difference in Cd concentration between the CaP and BPH groups. Immunoblots from both groups revealed a single band. The relative intensity of the MT band was 0.58 ± 0.09 in the BPH group and 0.17 ± 0.03 in the CaP group. MT expression in patients with BPH was 3.4-fold higher than in those with CaP.
Conclusion: MT may bind heavy metals and protect patients from CaP. Additional studies are needed to reveal the factors that influence the expression of MT in prostate epithelial cells, and to analyze the free and compound forms of Cd at the same time. |
topic |
benign prostatic hyperplasia cadmium metallothionein prostate cancer |
url |
http://www.sciencedirect.com/science/article/pii/S0929664609603739 |
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