GAP-43 expression correlates with spinal motoneuron regeneration following root avulsion

• Main Authors: So Kwok-Fai, Su Huanxing, Hu Bing, Yuan Qiuju, Lin Zhixiu, Wu Wutian
• Format: Article
• Language: English
• Published: Georg Thieme Verlag KG 2009-10-01
• Series: Journal of Brachial Plexus and Peripheral Nerve Injury
• Online Access: http://www.jbppni.com/content/4/1/18

<p>Abstract</p> <p>Background</p> <p>The growth-associated protein GAP-43 plays a crucial role in axonal regeneration in injured neurons.</p> <p>Methods</p> <p>We have used immunohistochemistry to investigate the expression of GAP-43 in spinal motoneurons during nerve reconstruction following root avulsion in the neonatal and adult rats.</p> <p>Results</p> <p>Following the injury, GAP-43-immunoreactivity (IR) could be found in adult avulsed motoneurons as early as 1 day, increased from 3 to 7 days and reached a maximal level at 2 weeks post-injury. The up-regulation of GAP-43 in adult avulsed motoneurons was accompanied with the axonal regeneration indicated by numerous regenerating motor axons entering the reimplanted ventral root and nerve. In contrast, GAP-43-IR could not be found in the neonatal avulsed motoneurons at any examined post-injury time points. This failure of up-regulation of GAP-43 was coincident with no axonal regeneration in the reimplanted nerve in the neonatal rats.</p> <p>Conclusion</p> <p>Close association of GAP-43 expression and capacity of regeneration in reimplanted spinal nerve of avulsed motoneurons suggests that GAP-43 is a potential therapeutic target for treatment of root avulsion of brachial plexus.</p>