Determination the Lipase Activity of Staphylococcus sp. Strain Isolated from Clinical Specimens
Bacterial lipases are the most important collection of biocatalysts used for a variety of biotechnological applications. In the current study the morphological, biochemical and Molecular characteristics to the extracellular lipase producing bacteria were identified. The bacterial isolates were sel...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Journal of Pure and Applied Microbiology
2020-03-01
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| Series: | Journal of Pure and Applied Microbiology |
| Subjects: | |
| Online Access: | https://microbiologyjournal.org/determination-the-lipase-activity-of-staphylococcus-sp-strain-isolated-from-clinical-specimens/ |
| Summary: | Bacterial lipases are the most important collection of biocatalysts used for a variety of biotechnological
applications. In the current study the morphological, biochemical and Molecular characteristics to the
extracellular lipase producing bacteria were identified. The bacterial isolates were selected as lipase
producing bacteria using Rhodamine-B agar plate. the production of Lipase from the organism were
determined with varying incubation temperature (20 to 50°C) and range of incubation time (16-120)
hrs. 16 bacterial isolates were identified as Staphylococcus hemolyticus strain SHKS1 with Genbank
acc. MK977614.1. By comparing the observed DNA sequences of these local samples with the retrieved
DNA sequences (GenBank acc. MG595372.1) as showed high lipase activity according to experimental
conditions about 140 U/ml. The current study showed that enzymatic activity of crude lipase extract
was maximum in 72 hrs incubation period and 37°C. |
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| ISSN: | 0973-7510 2581-690X |