| Summary: | Glaciers have been recognized as biomes, dominated by microbial life. Many novel species have been isolated from glacier ecosystems, and their physiological features are well characterized. However, genomic features of bacteria isolated from the deep ice core are poorly understood. In this study, we performed a comparative genomic analysis to uncover the genomic features of strain <i>Dyadobacter tibetensis</i> Y620-1 isolated from a 59 m depth of the ice core drilled from a Tibetan Plateau glacier. Strain <i>D. tibetensis</i> Y620-1 had the smallest genome among the 12 cultured <i>Dyadobacter</i> strains, relatively low GC content, and was placed at the root position of the phylogenomic tree. The gene family based on a nonmetric multidimensional scaling (NMDS) plot revealed a clear separation of strain <i>D. tibetensis</i> Y620-1 from the reference strains. The genome of the deep ice core isolated strain contained the highest percentage of new genes. The definitive difference is that all genes required for the serine-glyoxylate cycle in one-carbon metabolism were only found in strain <i>D. tibetensis</i> Y620-1, but not in any of the reference strains. The placement of strain <i>D. tibetensis</i> Y620-1 in the root of the phylogenomic tree suggests that these new genes and functions are of ancient origin. All of these genomic features may contribute to the survival of <i>D. tibetensis</i> Y620-1 in the glacier.
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