Investigation of Interleukin -18 Gene Polymorphisms and Serum Level in Patients with Preeclampsia

• Main Authors: M Solimanipour, S Naeimi, N Erfani
• Format: Article
• Language: fas
• Published: Yasuj University Of Medical Sciences 2011-10-01
• Series: Armaghane Danesh Bimonthly Journal
• Subjects: IL-18; Polymorphism; Preeclampsia; Genotype
• Online Access: http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-202&slc_lang=en&sid=1

Background & Aim: Preeclampsia is one of the most important factors in fetal and maternal mortality that seems to change immunological parameters such as rate of auto antibodies and cytokines involved in the disease. This study aimed to evaluate the serum level and frequency of the two polymorphisms in the IL-18 gene promoter in patients with preeclampsia and normal pregnant women. Methods: In the present case-control study conducted at Islamic Azad University of Estahban in 2009-2010, fifty preeclampsia patients and 103 healthy pregnant women were enrolled. Single nucleotide polymorphisms of the IL-18 gene at positions -607 (C/A) and -137 (G/C) were analyzed by the Allel specific PCR method. IL-18 serum level was determined, using ELISA method. Data were analyzed with chi-square tests, Mann-Whitney and the Hardy Weinberg equilibrium. Results: No significant association was found between the serum level, allele, genotype, and haplotype distributions of the SNPs and preeclampsia (p>0.05). Conclusion: Results of this study showed that IL-18 gene promoter polymorphisms at positions -607 and -137 and serum level did not confer susceptibility to preeclampsia in patients.