Application and Optimization of relE as a Negative Selection Marker for Making Definitive Genetic Constructs in Uropathogenic Escherichia coli

Application and Optimization of relE as a Negative Selection Marker for Making Definitive Genetic Constructs in Uropathogenic Escherichia coli

Studies of Uropathogenic Escherichia coli (UPEC) pathogenesis have relied heavily on genetic manipulation to understand virulence factors. We applied a recently reported positive-negative selection system to create a series of unmarked, scarless FimH mutants that show identical phenotypes to previou...

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Bibliographic Details
Main Authors: Varnica Khetrapal, Kurosh S. Mehershahi, Siyi Chen, Swaine L. Chen
Format: Article
Language:English
Published: MDPI AG 2016-01-01
Series:Pathogens
Subjects:
negative selection
UTI89
FimH
Online Access:http://www.mdpi.com/2076-0817/5/1/9

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