Preparation of Rat Gingival Mitochondria with an Improved Isolation Method
In order to establish a method of obtaining rat gingival mitochondria (Mt), Mt fractions were prepared in various combinations of homogenizing time with collagenase concentration. Rat gingival tissues were excised, minced, treated with collagenase, homogenized, and subjected to differential centri...
Main Authors: | Noriaki Kaneko, Tetsuya Rikimaru, Tetsuyuki Fujimura, Shigeyasu Mori, Saburo Hidaka, Hidehiro Kaya |
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Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2010-01-01
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Series: | International Journal of Dentistry |
Online Access: | http://dx.doi.org/10.1155/2010/275103 |
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