Preparation of Rat Gingival Mitochondria with an Improved Isolation Method

Preparation of Rat Gingival Mitochondria with an Improved Isolation Method

In order to establish a method of obtaining rat gingival mitochondria (Mt), Mt fractions were prepared in various combinations of homogenizing time with collagenase concentration. Rat gingival tissues were excised, minced, treated with collagenase, homogenized, and subjected to differential centri...

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Main Authors: Noriaki Kaneko, Tetsuya Rikimaru, Tetsuyuki Fujimura, Shigeyasu Mori, Saburo Hidaka, Hidehiro Kaya
Format: Article
Language:English
Published: Hindawi Limited 2010-01-01
Series:International Journal of Dentistry
Online Access:http://dx.doi.org/10.1155/2010/275103
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spelling doaj-8d3c3d28f2f74bd084671243f7dad0af2020-11-25T01:05:25ZengHindawi LimitedInternational Journal of Dentistry1687-87281687-87362010-01-01201010.1155/2010/275103275103Preparation of Rat Gingival Mitochondria with an Improved Isolation MethodNoriaki Kaneko0Tetsuya Rikimaru1Tetsuyuki Fujimura2Shigeyasu Mori3Saburo Hidaka4Hidehiro Kaya5Department of Dental Hygiene, Fukuoka College of Health Sciences, 2-15-1 Tamura, Sawara-Ku, Fukuoka 814-0193, JapanDepartment of Dental Hygiene, Fukuoka College of Health Sciences, 2-15-1 Tamura, Sawara-Ku, Fukuoka 814-0193, JapanFujimura Clinic, Fukuoka, JapanHamadayama Smile Dental Clinic, Tokyo, JapanDepartment of Dental Hygiene, Fukuoka College of Health Sciences, 2-15-1 Tamura, Sawara-Ku, Fukuoka 814-0193, JapanDepartment of Dental Hygiene, Fukuoka College of Health Sciences, 2-15-1 Tamura, Sawara-Ku, Fukuoka 814-0193, JapanIn order to establish a method of obtaining rat gingival mitochondria (Mt), Mt fractions were prepared in various combinations of homogenizing time with collagenase concentration. Rat gingival tissues were excised, minced, treated with collagenase, homogenized, and subjected to differential centrifugation rates. Both the respiratory control ratio (RCR) and adenosine diphosphate/oxygen (ADP/O) ratio of the Mt fraction prepared in a combination of 40, 50, or 60 sec homogenization with collagenase in a concentration range of 0.115%–0.130% (w/v) were measured. The values for the RCR and ADP/O ratio of the Mt fraction obtained in an optimal condition was 1.80±0.05 and 1.65±0.03, respectively. These results suggest that Mt of fairly high quality can be obtained through this refined combination of the homogenizing time and collagenase concentration.http://dx.doi.org/10.1155/2010/275103
collection DOAJ
language English
format Article
sources DOAJ
author Noriaki Kaneko
Tetsuya Rikimaru
Tetsuyuki Fujimura
Shigeyasu Mori
Saburo Hidaka
Hidehiro Kaya
spellingShingle Noriaki Kaneko
Tetsuya Rikimaru
Tetsuyuki Fujimura
Shigeyasu Mori
Saburo Hidaka
Hidehiro Kaya
Preparation of Rat Gingival Mitochondria with an Improved Isolation Method
International Journal of Dentistry
author_facet Noriaki Kaneko
Tetsuya Rikimaru
Tetsuyuki Fujimura
Shigeyasu Mori
Saburo Hidaka
Hidehiro Kaya
author_sort Noriaki Kaneko
title Preparation of Rat Gingival Mitochondria with an Improved Isolation Method
title_short Preparation of Rat Gingival Mitochondria with an Improved Isolation Method
title_full Preparation of Rat Gingival Mitochondria with an Improved Isolation Method
title_fullStr Preparation of Rat Gingival Mitochondria with an Improved Isolation Method
title_full_unstemmed Preparation of Rat Gingival Mitochondria with an Improved Isolation Method
title_sort preparation of rat gingival mitochondria with an improved isolation method
publisher Hindawi Limited
series International Journal of Dentistry
issn 1687-8728
1687-8736
publishDate 2010-01-01
description In order to establish a method of obtaining rat gingival mitochondria (Mt), Mt fractions were prepared in various combinations of homogenizing time with collagenase concentration. Rat gingival tissues were excised, minced, treated with collagenase, homogenized, and subjected to differential centrifugation rates. Both the respiratory control ratio (RCR) and adenosine diphosphate/oxygen (ADP/O) ratio of the Mt fraction prepared in a combination of 40, 50, or 60 sec homogenization with collagenase in a concentration range of 0.115%–0.130% (w/v) were measured. The values for the RCR and ADP/O ratio of the Mt fraction obtained in an optimal condition was 1.80±0.05 and 1.65±0.03, respectively. These results suggest that Mt of fairly high quality can be obtained through this refined combination of the homogenizing time and collagenase concentration.
url http://dx.doi.org/10.1155/2010/275103
work_keys_str_mv AT noriakikaneko preparationofratgingivalmitochondriawithanimprovedisolationmethod
AT tetsuyarikimaru preparationofratgingivalmitochondriawithanimprovedisolationmethod
AT tetsuyukifujimura preparationofratgingivalmitochondriawithanimprovedisolationmethod
AT shigeyasumori preparationofratgingivalmitochondriawithanimprovedisolationmethod
AT saburohidaka preparationofratgingivalmitochondriawithanimprovedisolationmethod
AT hidehirokaya preparationofratgingivalmitochondriawithanimprovedisolationmethod
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