Spectral imaging toolbox: segmentation, hyperstack reconstruction, and batch processing of spectral images for the determination of cell and model membrane lipid order

Spectral imaging toolbox: segmentation, hyperstack reconstruction, and batch processing of spectral images for the determination of cell and model membrane lipid order

Abstract Background Spectral imaging with polarity-sensitive fluorescent probes enables the quantification of cell and model membrane physical properties, including local hydration, fluidity, and lateral lipid packing, usually characterized by the generalized polarization (GP) parameter. With the de...

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Bibliographic Details
Main Authors: Miles Aron, Richard Browning, Dario Carugo, Erdinc Sezgin, Jorge Bernardino de la Serna, Christian Eggeling, Eleanor Stride
Format: Article
Language:English
Published: BMC 2017-05-01
Series:BMC Bioinformatics
Subjects:
Spectral imaging
Lipid order
Lipid packing
Membrane viscosity
Membrane segmentation
Laurdan
Online Access:http://link.springer.com/article/10.1186/s12859-017-1656-2

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