Glucosinolate Profile of Croatian Stenoendemic Plant <i>Fibigia triquetra</i> (DC.) Boiss. ex Prantl.

• Main Authors: Ivica Blažević, Gina Rosalinda De Nicola, Sabine Montaut, Patrick Rollin, Mirko Ruščić
• Format: Article
• Language: English
• Published: Croatian Chemical Society 2015-12-01
• Series: Croatica Chemica Acta
• Online Access: http://hrcak.srce.hr/file/221514

As part of our ongoing investigation of the stenoendemic plants belonging to the Brassicaceae family, we report on the chemistry of <i>Fibigia triquetra</i> (DC.) Boiss. ex Prantl for the first time. Different plant parts (flower, leaf, stem, and seed) of <i>F. triquetra</i> were characterized and quantified for glucosinolates (GLs) according to the ISO 9167-1 EU official method based on the HPLC analysis of desulfo-GLs. A taxonomic screening showed that <i>F. triquetra</i> contained relatively high levels of C-4 GLs, namely but-3-enyl GL (gluconapin, <b>1a</b>), 4-methylsulfanylbutyl GL (glucoerucin, <b>3a</b>), and 4-methylsulfinylbutyl GL (glucoraphanin, <b>5a</b>). GC-MS analysis of the volatile fractions obtained after enzyme hydrolysis and/or HPLC-ESI-MS of intact GLs confirmed the GL profile. Four minor GLs, namely isopropyl GL (glucoputranjivin, <b>6a</b>), sec-butyl GL (glucocochlearin, <b>7a</b>), pent-4-enyl GL (glucobrassicanapin, <b>2a</b>), and 5-methylsulfanylpentyl GL (glucoberteroin, <b>4a</b>) were also identified and quantified while 4-methylpentyl GL, 5-methylhexyl GL, and <i>n</i>-heptyl GL, were tentatively identified by GC-MS of their degradation products. Based on the major, as well as the minor GLs, this study shows differences in chemotaxonomy between <i>F. triquetra</i> and the related Degenia velebitica (Degen) Hayek as well as other investigated species of the genus <i>Fibigia</i>.