Enhancing production of alkaline polygalacturonate lyase from Bacillus subtilis by fed-batch fermentation.

Enhancing production of alkaline polygalacturonate lyase from Bacillus subtilis by fed-batch fermentation.

Alkaline polygalacturonate lyase (PGL, EC 4.2.2.2) is an enzyme used in many industries. We developed a fed-batch fermentation process that combines the enzymatic pretreatment of the carbon source with controlling the pH of the fermentative broth to enhance the PGL production from Bacillus subtilis...

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Main Authors: Mouyong Zou, Fenfen Guo, Xuezhi Li, Jian Zhao, Yinbo Qu
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3946182?pdf=render
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spelling doaj-8cb0785f7d3e47de88b9eaf350ac1a372020-11-24T22:00:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0193e9039210.1371/journal.pone.0090392Enhancing production of alkaline polygalacturonate lyase from Bacillus subtilis by fed-batch fermentation.Mouyong ZouFenfen GuoXuezhi LiJian ZhaoYinbo QuAlkaline polygalacturonate lyase (PGL, EC 4.2.2.2) is an enzyme used in many industries. We developed a fed-batch fermentation process that combines the enzymatic pretreatment of the carbon source with controlling the pH of the fermentative broth to enhance the PGL production from Bacillus subtilis 7-3-3 to decrease the production cost. Maintaining the fermentation broth at pH 6.5 prior to feeding with ammonia and at pH 6.0 after feeding significantly improved PGL activity (743.5 U mL-1) compared with the control (202.5 U mL-1). The average PGL productivity reached 19.6 U mL-1 h-1 after 38 h of fermentation. The crude PGL was suitable for environmentally friendly ramie enzymatic degumming.http://europepmc.org/articles/PMC3946182?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Mouyong Zou
Fenfen Guo
Xuezhi Li
Jian Zhao
Yinbo Qu
spellingShingle Mouyong Zou
Fenfen Guo
Xuezhi Li
Jian Zhao
Yinbo Qu
Enhancing production of alkaline polygalacturonate lyase from Bacillus subtilis by fed-batch fermentation.
PLoS ONE
author_facet Mouyong Zou
Fenfen Guo
Xuezhi Li
Jian Zhao
Yinbo Qu
author_sort Mouyong Zou
title Enhancing production of alkaline polygalacturonate lyase from Bacillus subtilis by fed-batch fermentation.
title_short Enhancing production of alkaline polygalacturonate lyase from Bacillus subtilis by fed-batch fermentation.
title_full Enhancing production of alkaline polygalacturonate lyase from Bacillus subtilis by fed-batch fermentation.
title_fullStr Enhancing production of alkaline polygalacturonate lyase from Bacillus subtilis by fed-batch fermentation.
title_full_unstemmed Enhancing production of alkaline polygalacturonate lyase from Bacillus subtilis by fed-batch fermentation.
title_sort enhancing production of alkaline polygalacturonate lyase from bacillus subtilis by fed-batch fermentation.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2014-01-01
description Alkaline polygalacturonate lyase (PGL, EC 4.2.2.2) is an enzyme used in many industries. We developed a fed-batch fermentation process that combines the enzymatic pretreatment of the carbon source with controlling the pH of the fermentative broth to enhance the PGL production from Bacillus subtilis 7-3-3 to decrease the production cost. Maintaining the fermentation broth at pH 6.5 prior to feeding with ammonia and at pH 6.0 after feeding significantly improved PGL activity (743.5 U mL-1) compared with the control (202.5 U mL-1). The average PGL productivity reached 19.6 U mL-1 h-1 after 38 h of fermentation. The crude PGL was suitable for environmentally friendly ramie enzymatic degumming.
url http://europepmc.org/articles/PMC3946182?pdf=render
work_keys_str_mv AT mouyongzou enhancingproductionofalkalinepolygalacturonatelyasefrombacillussubtilisbyfedbatchfermentation
AT fenfenguo enhancingproductionofalkalinepolygalacturonatelyasefrombacillussubtilisbyfedbatchfermentation
AT xuezhili enhancingproductionofalkalinepolygalacturonatelyasefrombacillussubtilisbyfedbatchfermentation
AT jianzhao enhancingproductionofalkalinepolygalacturonatelyasefrombacillussubtilisbyfedbatchfermentation
AT yinboqu enhancingproductionofalkalinepolygalacturonatelyasefrombacillussubtilisbyfedbatchfermentation
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