Uso de equações lineares na determinação dos parâmetros de Michaelis-Menten Use of linear equations to obtain Michaelis-Menten parameters

Uso de equações lineares na determinação dos parâmetros de Michaelis-Menten Use of linear equations to obtain Michaelis-Menten parameters

<abstract language="eng">The Michaelis-Menten equation is used in many biochemical and bioinorganic kinetic studies involving homogeneous catalysis. Otherwise, it is known that determination of Michaelis-Menten parameters K M, Vmax, and k cat by the well-known Lineweaver-Burk double...

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Main Authors: Nakédia M. F. Carvalho, Bianca M. Pires, Octavio A. C. Antunes, Roberto B. Faria, Renata E. H. M. B. Osório, Clovis Piovezan, Ademir Neves
Format: Article
Language:English
Published: Sociedade Brasileira de Química 2010-01-01
Series:Química Nova
Subjects:
Michaelis-Menten
Lineweaver-Burk
nonlinear least-square fit
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000700033
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spelling doaj-8c6c97b18c3e41188b966e4bfe9012472020-11-24T22:09:17ZengSociedade Brasileira de QuímicaQuímica Nova0100-40421678-70642010-01-013371607161110.1590/S0100-40422010000700033Uso de equações lineares na determinação dos parâmetros de Michaelis-Menten Use of linear equations to obtain Michaelis-Menten parametersNakédia M. F. CarvalhoBianca M. PiresOctavio A. C. AntunesRoberto B. FariaRenata E. H. M. B. OsórioClovis PiovezanAdemir Neves<abstract language="eng">The Michaelis-Menten equation is used in many biochemical and bioinorganic kinetic studies involving homogeneous catalysis. Otherwise, it is known that determination of Michaelis-Menten parameters K M, Vmax, and k cat by the well-known Lineweaver-Burk double reciprocal linear equation does not produce the best values for these parameters. In this paper we present a discussion on different linear equations which can be used to calculate these parameters and we compare their results with the values obtained by the more reliable nonlinear least-square fit.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000700033Michaelis-MentenLineweaver-Burknonlinear least-square fit
collection DOAJ
language English
format Article
sources DOAJ
author Nakédia M. F. Carvalho
Bianca M. Pires
Octavio A. C. Antunes
Roberto B. Faria
Renata E. H. M. B. Osório
Clovis Piovezan
Ademir Neves
spellingShingle Nakédia M. F. Carvalho
Bianca M. Pires
Octavio A. C. Antunes
Roberto B. Faria
Renata E. H. M. B. Osório
Clovis Piovezan
Ademir Neves
Uso de equações lineares na determinação dos parâmetros de Michaelis-Menten Use of linear equations to obtain Michaelis-Menten parameters
Química Nova
Michaelis-Menten
Lineweaver-Burk
nonlinear least-square fit
author_facet Nakédia M. F. Carvalho
Bianca M. Pires
Octavio A. C. Antunes
Roberto B. Faria
Renata E. H. M. B. Osório
Clovis Piovezan
Ademir Neves
author_sort Nakédia M. F. Carvalho
title Uso de equações lineares na determinação dos parâmetros de Michaelis-Menten Use of linear equations to obtain Michaelis-Menten parameters
title_short Uso de equações lineares na determinação dos parâmetros de Michaelis-Menten Use of linear equations to obtain Michaelis-Menten parameters
title_full Uso de equações lineares na determinação dos parâmetros de Michaelis-Menten Use of linear equations to obtain Michaelis-Menten parameters
title_fullStr Uso de equações lineares na determinação dos parâmetros de Michaelis-Menten Use of linear equations to obtain Michaelis-Menten parameters
title_full_unstemmed Uso de equações lineares na determinação dos parâmetros de Michaelis-Menten Use of linear equations to obtain Michaelis-Menten parameters
title_sort uso de equações lineares na determinação dos parâmetros de michaelis-menten use of linear equations to obtain michaelis-menten parameters
publisher Sociedade Brasileira de Química
series Química Nova
issn 0100-4042
1678-7064
publishDate 2010-01-01
description <abstract language="eng">The Michaelis-Menten equation is used in many biochemical and bioinorganic kinetic studies involving homogeneous catalysis. Otherwise, it is known that determination of Michaelis-Menten parameters K M, Vmax, and k cat by the well-known Lineweaver-Burk double reciprocal linear equation does not produce the best values for these parameters. In this paper we present a discussion on different linear equations which can be used to calculate these parameters and we compare their results with the values obtained by the more reliable nonlinear least-square fit.
topic Michaelis-Menten
Lineweaver-Burk
nonlinear least-square fit
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000700033
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