Increased ARPP-19 Expression Is Associated with Hepatocellular Carcinoma

The cAMP-regulated phosphoprotein 19 (ARPP-19) plays a key role in cell mitotic G2/M transition. Expression of ARPP-19 was increased in human hepatocellular carcinoma (HCC) compared to adjacent non-tumorous liver tissues in 36 paired liver samples, and the level of ARPP-19 in HCC tissues was positiv...

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Main Authors:	Haiyan Song, Jielu Pan, Yang Liu, Hongzhu Wen, Lei Wang, Jiefeng Cui, Yinkun Liu, Bing Hu, Zemin Yao, Guang Ji
Format:	Article
Language:	English
Published:	MDPI AG 2014-12-01
Series:	International Journal of Molecular Sciences
Subjects:	hepatocellular carcinoma ARPP-19 cell proliferation cell cycle
Online Access:	http://www.mdpi.com/1422-0067/16/1/178

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spelling	doaj-8c293cbebaf1446389e0a9219e25890b2020-11-25T01:18:05ZengMDPI AGInternational Journal of Molecular Sciences1422-00672014-12-0116117819210.3390/ijms16010178ijms16010178Increased ARPP-19 Expression Is Associated with Hepatocellular CarcinomaHaiyan Song0Jielu Pan1Yang Liu2Hongzhu Wen3Lei Wang4Jiefeng Cui5Yinkun Liu6Bing Hu7Zemin Yao8Guang Ji9Institute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, ChinaInstitute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, ChinaInstitute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, ChinaInstitute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, ChinaInstitute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, ChinaLiver Cancer Institute of Zhongshan Hospital, Fudan University, Shanghai 200032, ChinaLiver Cancer Institute of Zhongshan Hospital, Fudan University, Shanghai 200032, ChinaDepartment of Oncology and Institute of Traditional Chinese Medicine in Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, ChinaDepartment of Biochemistry, Microbiology and Immunology, Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, ON K1H 8M5, CanadaInstitute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, ChinaThe cAMP-regulated phosphoprotein 19 (ARPP-19) plays a key role in cell mitotic G2/M transition. Expression of ARPP-19 was increased in human hepatocellular carcinoma (HCC) compared to adjacent non-tumorous liver tissues in 36 paired liver samples, and the level of ARPP-19 in HCC tissues was positively correlated with the tumor size. To determine the interrelationship between ARPP-19 expression and HCC, we silenced ARPP-19 expression in the human hepatocarcinoma HepG2 and SMMC-7721 cells using lentivirus encoding ARPP-19 siRNA. HepG2 and SMMC-7721 cells with ARPP-19 knockdown displayed lowered cell growth rate, retarded colony formation and increased arrest at the G2/M phase transition. Silencing ARPP-19 in HCC cells resulted in decreased protein levels of phospho-(Ser) CDKs substrates and increased levels of inactivated cyclin division cycle 2 (Cdc2). Therefore, ARPP-19 may play a role in HCC pathogenesis through regulating cell proliferation.http://www.mdpi.com/1422-0067/16/1/178hepatocellular carcinomaARPP-19cell proliferationcell cycle
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Haiyan Song Jielu Pan Yang Liu Hongzhu Wen Lei Wang Jiefeng Cui Yinkun Liu Bing Hu Zemin Yao Guang Ji
spellingShingle	Haiyan Song Jielu Pan Yang Liu Hongzhu Wen Lei Wang Jiefeng Cui Yinkun Liu Bing Hu Zemin Yao Guang Ji Increased ARPP-19 Expression Is Associated with Hepatocellular Carcinoma International Journal of Molecular Sciences hepatocellular carcinoma ARPP-19 cell proliferation cell cycle
author_facet	Haiyan Song Jielu Pan Yang Liu Hongzhu Wen Lei Wang Jiefeng Cui Yinkun Liu Bing Hu Zemin Yao Guang Ji
author_sort	Haiyan Song
title	Increased ARPP-19 Expression Is Associated with Hepatocellular Carcinoma
title_short	Increased ARPP-19 Expression Is Associated with Hepatocellular Carcinoma
title_full	Increased ARPP-19 Expression Is Associated with Hepatocellular Carcinoma
title_fullStr	Increased ARPP-19 Expression Is Associated with Hepatocellular Carcinoma
title_full_unstemmed	Increased ARPP-19 Expression Is Associated with Hepatocellular Carcinoma
title_sort	increased arpp-19 expression is associated with hepatocellular carcinoma
publisher	MDPI AG
series	International Journal of Molecular Sciences
issn	1422-0067
publishDate	2014-12-01
description	The cAMP-regulated phosphoprotein 19 (ARPP-19) plays a key role in cell mitotic G2/M transition. Expression of ARPP-19 was increased in human hepatocellular carcinoma (HCC) compared to adjacent non-tumorous liver tissues in 36 paired liver samples, and the level of ARPP-19 in HCC tissues was positively correlated with the tumor size. To determine the interrelationship between ARPP-19 expression and HCC, we silenced ARPP-19 expression in the human hepatocarcinoma HepG2 and SMMC-7721 cells using lentivirus encoding ARPP-19 siRNA. HepG2 and SMMC-7721 cells with ARPP-19 knockdown displayed lowered cell growth rate, retarded colony formation and increased arrest at the G2/M phase transition. Silencing ARPP-19 in HCC cells resulted in decreased protein levels of phospho-(Ser) CDKs substrates and increased levels of inactivated cyclin division cycle 2 (Cdc2). Therefore, ARPP-19 may play a role in HCC pathogenesis through regulating cell proliferation.
topic	hepatocellular carcinoma ARPP-19 cell proliferation cell cycle
url	http://www.mdpi.com/1422-0067/16/1/178
work_keys_str_mv	AT haiyansong increasedarpp19expressionisassociatedwithhepatocellularcarcinoma AT jielupan increasedarpp19expressionisassociatedwithhepatocellularcarcinoma AT yangliu increasedarpp19expressionisassociatedwithhepatocellularcarcinoma AT hongzhuwen increasedarpp19expressionisassociatedwithhepatocellularcarcinoma AT leiwang increasedarpp19expressionisassociatedwithhepatocellularcarcinoma AT jiefengcui increasedarpp19expressionisassociatedwithhepatocellularcarcinoma AT yinkunliu increasedarpp19expressionisassociatedwithhepatocellularcarcinoma AT binghu increasedarpp19expressionisassociatedwithhepatocellularcarcinoma AT zeminyao increasedarpp19expressionisassociatedwithhepatocellularcarcinoma AT guangji increasedarpp19expressionisassociatedwithhepatocellularcarcinoma
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Increased ARPP-19 Expression Is Associated with Hepatocellular Carcinoma

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