One-step PCR detection of Salmonella Pullorum/Gallinarum using a novel target: the flagellar biosynthesis gene flhB
Salmonella enterica serovar Pullorum/Gallinarum is an important infectious pathogen that has caused widespread problems for chicken industry. Traditional Salmonella serotyping is an expensive and time-consuming process. In this study, we developed a rapid one-step PCR method to identify S. Pullorum/...
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2016-11-01
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doaj-8c167c8b465548d99feb46c804a3fb722020-11-24T20:45:53ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-11-01710.3389/fmicb.2016.01863229332One-step PCR detection of Salmonella Pullorum/Gallinarum using a novel target: the flagellar biosynthesis gene flhBDan Xiong0Dan Xiong1Dan Xiong2Li Song3Li Song4Li Song5Shizhong Geng6Shizhong Geng7Shizhong Geng8Jing Tao9Jing Tao10Jing Tao11Shumin An12Shumin An13Shumin An14Zhiming Pan15Zhiming Pan16Zhiming Pan17Xinan Jiao18Xinan Jiao19Xinan Jiao20Yangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesJoint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of EducationYangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesJoint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of EducationYangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesJoint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of EducationYangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesJoint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of EducationYangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesJoint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of EducationYangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesJoint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of EducationYangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesJoint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of EducationSalmonella enterica serovar Pullorum/Gallinarum is an important infectious pathogen that has caused widespread problems for chicken industry. Traditional Salmonella serotyping is an expensive and time-consuming process. In this study, we developed a rapid one-step PCR method to identify S. Pullorum/Gallinarum. The PCR-based assay focuses on flhB, which shows a deficient region only in S. Pullorum/Gallinarum, compared with that of other serovars. The specificity and sensitivity of the PCR system were evaluated. The developed PCR method could identify S. Pullorum/Gallinarum from 27 different Salmonella serovars and eight non-Salmonella pathogens. The minimum limit of DNA and the lowest number of cells of S. Pullorum for the PCR detection were no less than 5.85 pg/μL and 10 CFU, respectively. The method was applied to the analysis of Salmonella strains isolated from the chicken farm. The PCR-based testing results of the farm isolates were in concordance with those obtained using traditional serotyping method. This newly developed PCR-based system could be used to accurately screen for the presence of S. Pullorum/Gallinarum, and support traditional serotyping methods, especially in high-throughput screening situations.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01863/fullChicken farmOne stepPCR detectionFlhBSalmonella Pullorum/Gallinarum |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dan Xiong Dan Xiong Dan Xiong Li Song Li Song Li Song Shizhong Geng Shizhong Geng Shizhong Geng Jing Tao Jing Tao Jing Tao Shumin An Shumin An Shumin An Zhiming Pan Zhiming Pan Zhiming Pan Xinan Jiao Xinan Jiao Xinan Jiao |
spellingShingle |
Dan Xiong Dan Xiong Dan Xiong Li Song Li Song Li Song Shizhong Geng Shizhong Geng Shizhong Geng Jing Tao Jing Tao Jing Tao Shumin An Shumin An Shumin An Zhiming Pan Zhiming Pan Zhiming Pan Xinan Jiao Xinan Jiao Xinan Jiao One-step PCR detection of Salmonella Pullorum/Gallinarum using a novel target: the flagellar biosynthesis gene flhB Frontiers in Microbiology Chicken farm One step PCR detection FlhB Salmonella Pullorum/Gallinarum |
author_facet |
Dan Xiong Dan Xiong Dan Xiong Li Song Li Song Li Song Shizhong Geng Shizhong Geng Shizhong Geng Jing Tao Jing Tao Jing Tao Shumin An Shumin An Shumin An Zhiming Pan Zhiming Pan Zhiming Pan Xinan Jiao Xinan Jiao Xinan Jiao |
author_sort |
Dan Xiong |
title |
One-step PCR detection of Salmonella Pullorum/Gallinarum using a novel target: the flagellar biosynthesis gene flhB |
title_short |
One-step PCR detection of Salmonella Pullorum/Gallinarum using a novel target: the flagellar biosynthesis gene flhB |
title_full |
One-step PCR detection of Salmonella Pullorum/Gallinarum using a novel target: the flagellar biosynthesis gene flhB |
title_fullStr |
One-step PCR detection of Salmonella Pullorum/Gallinarum using a novel target: the flagellar biosynthesis gene flhB |
title_full_unstemmed |
One-step PCR detection of Salmonella Pullorum/Gallinarum using a novel target: the flagellar biosynthesis gene flhB |
title_sort |
one-step pcr detection of salmonella pullorum/gallinarum using a novel target: the flagellar biosynthesis gene flhb |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2016-11-01 |
description |
Salmonella enterica serovar Pullorum/Gallinarum is an important infectious pathogen that has caused widespread problems for chicken industry. Traditional Salmonella serotyping is an expensive and time-consuming process. In this study, we developed a rapid one-step PCR method to identify S. Pullorum/Gallinarum. The PCR-based assay focuses on flhB, which shows a deficient region only in S. Pullorum/Gallinarum, compared with that of other serovars. The specificity and sensitivity of the PCR system were evaluated. The developed PCR method could identify S. Pullorum/Gallinarum from 27 different Salmonella serovars and eight non-Salmonella pathogens. The minimum limit of DNA and the lowest number of cells of S. Pullorum for the PCR detection were no less than 5.85 pg/μL and 10 CFU, respectively. The method was applied to the analysis of Salmonella strains isolated from the chicken farm. The PCR-based testing results of the farm isolates were in concordance with those obtained using traditional serotyping method. This newly developed PCR-based system could be used to accurately screen for the presence of S. Pullorum/Gallinarum, and support traditional serotyping methods, especially in high-throughput screening situations. |
topic |
Chicken farm One step PCR detection FlhB Salmonella Pullorum/Gallinarum |
url |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01863/full |
work_keys_str_mv |
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