One-step PCR detection of Salmonella Pullorum/Gallinarum using a novel target: the flagellar biosynthesis gene flhB

Salmonella enterica serovar Pullorum/Gallinarum is an important infectious pathogen that has caused widespread problems for chicken industry. Traditional Salmonella serotyping is an expensive and time-consuming process. In this study, we developed a rapid one-step PCR method to identify S. Pullorum/...

Full description

Bibliographic Details
Main Authors: Dan Xiong, Li Song, Shizhong Geng, Jing Tao, Shumin An, Zhiming Pan, Xinan Jiao
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-11-01
Series:Frontiers in Microbiology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01863/full
id doaj-8c167c8b465548d99feb46c804a3fb72
record_format Article
spelling doaj-8c167c8b465548d99feb46c804a3fb722020-11-24T20:45:53ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-11-01710.3389/fmicb.2016.01863229332One-step PCR detection of Salmonella Pullorum/Gallinarum using a novel target: the flagellar biosynthesis gene flhBDan Xiong0Dan Xiong1Dan Xiong2Li Song3Li Song4Li Song5Shizhong Geng6Shizhong Geng7Shizhong Geng8Jing Tao9Jing Tao10Jing Tao11Shumin An12Shumin An13Shumin An14Zhiming Pan15Zhiming Pan16Zhiming Pan17Xinan Jiao18Xinan Jiao19Xinan Jiao20Yangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesJoint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of EducationYangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesJoint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of EducationYangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesJoint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of EducationYangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesJoint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of EducationYangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesJoint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of EducationYangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesJoint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of EducationYangzhou UniversityJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesJoint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of EducationSalmonella enterica serovar Pullorum/Gallinarum is an important infectious pathogen that has caused widespread problems for chicken industry. Traditional Salmonella serotyping is an expensive and time-consuming process. In this study, we developed a rapid one-step PCR method to identify S. Pullorum/Gallinarum. The PCR-based assay focuses on flhB, which shows a deficient region only in S. Pullorum/Gallinarum, compared with that of other serovars. The specificity and sensitivity of the PCR system were evaluated. The developed PCR method could identify S. Pullorum/Gallinarum from 27 different Salmonella serovars and eight non-Salmonella pathogens. The minimum limit of DNA and the lowest number of cells of S. Pullorum for the PCR detection were no less than 5.85 pg/μL and 10 CFU, respectively. The method was applied to the analysis of Salmonella strains isolated from the chicken farm. The PCR-based testing results of the farm isolates were in concordance with those obtained using traditional serotyping method. This newly developed PCR-based system could be used to accurately screen for the presence of S. Pullorum/Gallinarum, and support traditional serotyping methods, especially in high-throughput screening situations.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01863/fullChicken farmOne stepPCR detectionFlhBSalmonella Pullorum/Gallinarum
collection DOAJ
language English
format Article
sources DOAJ
author Dan Xiong
Dan Xiong
Dan Xiong
Li Song
Li Song
Li Song
Shizhong Geng
Shizhong Geng
Shizhong Geng
Jing Tao
Jing Tao
Jing Tao
Shumin An
Shumin An
Shumin An
Zhiming Pan
Zhiming Pan
Zhiming Pan
Xinan Jiao
Xinan Jiao
Xinan Jiao
spellingShingle Dan Xiong
Dan Xiong
Dan Xiong
Li Song
Li Song
Li Song
Shizhong Geng
Shizhong Geng
Shizhong Geng
Jing Tao
Jing Tao
Jing Tao
Shumin An
Shumin An
Shumin An
Zhiming Pan
Zhiming Pan
Zhiming Pan
Xinan Jiao
Xinan Jiao
Xinan Jiao
One-step PCR detection of Salmonella Pullorum/Gallinarum using a novel target: the flagellar biosynthesis gene flhB
Frontiers in Microbiology
Chicken farm
One step
PCR detection
FlhB
Salmonella Pullorum/Gallinarum
author_facet Dan Xiong
Dan Xiong
Dan Xiong
Li Song
Li Song
Li Song
Shizhong Geng
Shizhong Geng
Shizhong Geng
Jing Tao
Jing Tao
Jing Tao
Shumin An
Shumin An
Shumin An
Zhiming Pan
Zhiming Pan
Zhiming Pan
Xinan Jiao
Xinan Jiao
Xinan Jiao
author_sort Dan Xiong
title One-step PCR detection of Salmonella Pullorum/Gallinarum using a novel target: the flagellar biosynthesis gene flhB
title_short One-step PCR detection of Salmonella Pullorum/Gallinarum using a novel target: the flagellar biosynthesis gene flhB
title_full One-step PCR detection of Salmonella Pullorum/Gallinarum using a novel target: the flagellar biosynthesis gene flhB
title_fullStr One-step PCR detection of Salmonella Pullorum/Gallinarum using a novel target: the flagellar biosynthesis gene flhB
title_full_unstemmed One-step PCR detection of Salmonella Pullorum/Gallinarum using a novel target: the flagellar biosynthesis gene flhB
title_sort one-step pcr detection of salmonella pullorum/gallinarum using a novel target: the flagellar biosynthesis gene flhb
publisher Frontiers Media S.A.
series Frontiers in Microbiology
issn 1664-302X
publishDate 2016-11-01
description Salmonella enterica serovar Pullorum/Gallinarum is an important infectious pathogen that has caused widespread problems for chicken industry. Traditional Salmonella serotyping is an expensive and time-consuming process. In this study, we developed a rapid one-step PCR method to identify S. Pullorum/Gallinarum. The PCR-based assay focuses on flhB, which shows a deficient region only in S. Pullorum/Gallinarum, compared with that of other serovars. The specificity and sensitivity of the PCR system were evaluated. The developed PCR method could identify S. Pullorum/Gallinarum from 27 different Salmonella serovars and eight non-Salmonella pathogens. The minimum limit of DNA and the lowest number of cells of S. Pullorum for the PCR detection were no less than 5.85 pg/μL and 10 CFU, respectively. The method was applied to the analysis of Salmonella strains isolated from the chicken farm. The PCR-based testing results of the farm isolates were in concordance with those obtained using traditional serotyping method. This newly developed PCR-based system could be used to accurately screen for the presence of S. Pullorum/Gallinarum, and support traditional serotyping methods, especially in high-throughput screening situations.
topic Chicken farm
One step
PCR detection
FlhB
Salmonella Pullorum/Gallinarum
url http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01863/full
work_keys_str_mv AT danxiong onesteppcrdetectionofsalmonellapullorumgallinarumusinganoveltargettheflagellarbiosynthesisgeneflhb
AT danxiong onesteppcrdetectionofsalmonellapullorumgallinarumusinganoveltargettheflagellarbiosynthesisgeneflhb
AT danxiong onesteppcrdetectionofsalmonellapullorumgallinarumusinganoveltargettheflagellarbiosynthesisgeneflhb
AT lisong onesteppcrdetectionofsalmonellapullorumgallinarumusinganoveltargettheflagellarbiosynthesisgeneflhb
AT lisong onesteppcrdetectionofsalmonellapullorumgallinarumusinganoveltargettheflagellarbiosynthesisgeneflhb
AT lisong onesteppcrdetectionofsalmonellapullorumgallinarumusinganoveltargettheflagellarbiosynthesisgeneflhb
AT shizhonggeng onesteppcrdetectionofsalmonellapullorumgallinarumusinganoveltargettheflagellarbiosynthesisgeneflhb
AT shizhonggeng onesteppcrdetectionofsalmonellapullorumgallinarumusinganoveltargettheflagellarbiosynthesisgeneflhb
AT shizhonggeng onesteppcrdetectionofsalmonellapullorumgallinarumusinganoveltargettheflagellarbiosynthesisgeneflhb
AT jingtao onesteppcrdetectionofsalmonellapullorumgallinarumusinganoveltargettheflagellarbiosynthesisgeneflhb
AT jingtao onesteppcrdetectionofsalmonellapullorumgallinarumusinganoveltargettheflagellarbiosynthesisgeneflhb
AT jingtao onesteppcrdetectionofsalmonellapullorumgallinarumusinganoveltargettheflagellarbiosynthesisgeneflhb
AT shuminan onesteppcrdetectionofsalmonellapullorumgallinarumusinganoveltargettheflagellarbiosynthesisgeneflhb
AT shuminan onesteppcrdetectionofsalmonellapullorumgallinarumusinganoveltargettheflagellarbiosynthesisgeneflhb
AT shuminan onesteppcrdetectionofsalmonellapullorumgallinarumusinganoveltargettheflagellarbiosynthesisgeneflhb
AT zhimingpan onesteppcrdetectionofsalmonellapullorumgallinarumusinganoveltargettheflagellarbiosynthesisgeneflhb
AT zhimingpan onesteppcrdetectionofsalmonellapullorumgallinarumusinganoveltargettheflagellarbiosynthesisgeneflhb
AT zhimingpan onesteppcrdetectionofsalmonellapullorumgallinarumusinganoveltargettheflagellarbiosynthesisgeneflhb
AT xinanjiao onesteppcrdetectionofsalmonellapullorumgallinarumusinganoveltargettheflagellarbiosynthesisgeneflhb
AT xinanjiao onesteppcrdetectionofsalmonellapullorumgallinarumusinganoveltargettheflagellarbiosynthesisgeneflhb
AT xinanjiao onesteppcrdetectionofsalmonellapullorumgallinarumusinganoveltargettheflagellarbiosynthesisgeneflhb
_version_ 1716813770330734592