Rapid Visual Tests: Fast and Reliable Detection of Ochratoxin A

• Main Authors: Miguel Lopez-Ferber, Elodie Nabais, Ingrid Bazin
• Format: Article
• Language: English
• Published: MDPI AG 2010-08-01
• Series: Toxins
• Subjects: ochratoxin A; colorimetric test; lateral flow test; flow-through test; clean-up immunoassay
• Online Access: http://www.mdpi.com/2072-6651/2/9/2230/

This paper reviews the early detection strategies that have been employed for the rapid monitoring of ochratoxin A (OTA) contamination of food. OTA, a mycotoxin mainly produced by some Aspergillus and Penicillium species, is found in cereals, coffee, wine, pork and grapes. To minimize the entry of this mycotoxin into the food chain, rapid diagnostic tools are required. To this end, the potential use of lateral flow devices has also been developed. In this study, we analyze the robustness of test strips using published methods for colorimetric detection. Different test formats are discussed, and challenges in the development of lateral flow devices for on-site determination of OTA, with requirements such as robustness, speed, and cost-effectiveness, are discussed.