The κB transcriptional enhancer motif and signal sequences of V(D)J recombination are targets for the zinc finger protein HIVEP3/KRC: a site selection amplification binding study

<p>Abstract</p> <p>Background</p> <p>The ZAS family is composed of proteins that regulate transcription via specific gene regulatory elements. The amino-DNA binding domain (ZAS-N) and the carboxyl-DNA binding domain (ZAS-C) of a representative family member, named κB DN...

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Main Authors: Wu Lai-Chu, Mak Chi-ho, Allen Carl E
Format: Article
Language:English
Published: BMC 2002-08-01
Series:BMC Immunology
Online Access:http://www.biomedcentral.com/1471-2172/3/10
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spelling doaj-8be4e83afbb943c0b6528d5293c3d16d2020-11-25T01:38:55ZengBMCBMC Immunology1471-21722002-08-01311010.1186/1471-2172-3-10The κB transcriptional enhancer motif and signal sequences of V(D)J recombination are targets for the zinc finger protein HIVEP3/KRC: a site selection amplification binding studyWu Lai-ChuMak Chi-hoAllen Carl E<p>Abstract</p> <p>Background</p> <p>The ZAS family is composed of proteins that regulate transcription via specific gene regulatory elements. The amino-DNA binding domain (ZAS-N) and the carboxyl-DNA binding domain (ZAS-C) of a representative family member, named κB DNA binding and recognition component (KRC), were expressed as fusion proteins and their target DNA sequences were elucidated by site selection amplification binding assays, followed by cloning and DNA sequencing. The fusion proteins-selected DNA sequences were analyzed by the MEME and MAST computer programs to obtain consensus motifs and DNA elements bound by the ZAS domains.</p> <p>Results</p> <p>Both fusion proteins selected sequences that were similar to the κB motif or the canonical elements of the V(D)J recombination signal sequences (RSS) from a pool of degenerate oligonucleotides. Specifically, the ZAS-N domain selected sequences similar to the canonical RSS nonamer, while ZAS-C domain selected sequences similar to the canonical RSS heptamer. In addition, both KRC fusion proteins selected oligonucleoties with sequences identical to heptamer and nonamer sequences within endogenous RSS.</p> <p>Conclusions</p> <p>The RSS are <it>cis</it>-acting DNA motifs which are essential for V(D)J recombination of antigen receptor genes. Due to its specific binding affinity for RSS and κB-like transcription enhancer motifs, we hypothesize that KRC may be involved in the regulation of V(D)J recombination.</p> http://www.biomedcentral.com/1471-2172/3/10
collection DOAJ
language English
format Article
sources DOAJ
author Wu Lai-Chu
Mak Chi-ho
Allen Carl E
spellingShingle Wu Lai-Chu
Mak Chi-ho
Allen Carl E
The κB transcriptional enhancer motif and signal sequences of V(D)J recombination are targets for the zinc finger protein HIVEP3/KRC: a site selection amplification binding study
BMC Immunology
author_facet Wu Lai-Chu
Mak Chi-ho
Allen Carl E
author_sort Wu Lai-Chu
title The κB transcriptional enhancer motif and signal sequences of V(D)J recombination are targets for the zinc finger protein HIVEP3/KRC: a site selection amplification binding study
title_short The κB transcriptional enhancer motif and signal sequences of V(D)J recombination are targets for the zinc finger protein HIVEP3/KRC: a site selection amplification binding study
title_full The κB transcriptional enhancer motif and signal sequences of V(D)J recombination are targets for the zinc finger protein HIVEP3/KRC: a site selection amplification binding study
title_fullStr The κB transcriptional enhancer motif and signal sequences of V(D)J recombination are targets for the zinc finger protein HIVEP3/KRC: a site selection amplification binding study
title_full_unstemmed The κB transcriptional enhancer motif and signal sequences of V(D)J recombination are targets for the zinc finger protein HIVEP3/KRC: a site selection amplification binding study
title_sort κb transcriptional enhancer motif and signal sequences of v(d)j recombination are targets for the zinc finger protein hivep3/krc: a site selection amplification binding study
publisher BMC
series BMC Immunology
issn 1471-2172
publishDate 2002-08-01
description <p>Abstract</p> <p>Background</p> <p>The ZAS family is composed of proteins that regulate transcription via specific gene regulatory elements. The amino-DNA binding domain (ZAS-N) and the carboxyl-DNA binding domain (ZAS-C) of a representative family member, named κB DNA binding and recognition component (KRC), were expressed as fusion proteins and their target DNA sequences were elucidated by site selection amplification binding assays, followed by cloning and DNA sequencing. The fusion proteins-selected DNA sequences were analyzed by the MEME and MAST computer programs to obtain consensus motifs and DNA elements bound by the ZAS domains.</p> <p>Results</p> <p>Both fusion proteins selected sequences that were similar to the κB motif or the canonical elements of the V(D)J recombination signal sequences (RSS) from a pool of degenerate oligonucleotides. Specifically, the ZAS-N domain selected sequences similar to the canonical RSS nonamer, while ZAS-C domain selected sequences similar to the canonical RSS heptamer. In addition, both KRC fusion proteins selected oligonucleoties with sequences identical to heptamer and nonamer sequences within endogenous RSS.</p> <p>Conclusions</p> <p>The RSS are <it>cis</it>-acting DNA motifs which are essential for V(D)J recombination of antigen receptor genes. Due to its specific binding affinity for RSS and κB-like transcription enhancer motifs, we hypothesize that KRC may be involved in the regulation of V(D)J recombination.</p>
url http://www.biomedcentral.com/1471-2172/3/10
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