Transfusion associated peak in Hb HPLC chromatogram – a case report

Transfusion associated peak in Hb HPLC chromatogram – a case report

High performance liquid chromatography (HPLC) and electrophoresis are commonly used to diagnose various hemoglobinopathies. However, insufficient information about the transfusion history can lead to unexpected and confusing results. We are reporting a case of Juvenile myelomonocytic leukemia (JMML)...

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Bibliographic Details
Main Authors: Sonal Jain, Jasmita Dass, Hara Prasad Pati
Format: Article
Language:English
Published: PAGEPress Publications 2012-01-01
Series:Mediterranean Journal of Hematology and Infectious Diseases
Subjects:
HPLC, Transfusion, HbD
Online Access:http://www.mjhid.org/index.php/mjhid/article/view/382
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spelling doaj-8b8d630193c94488ae3ea2fd0551157d2020-11-24T20:51:53ZengPAGEPress PublicationsMediterranean Journal of Hematology and Infectious Diseases2035-30062012-01-0141e2012006e201200610.4084/mjhid.2012.006274Transfusion associated peak in Hb HPLC chromatogram – a case reportSonal Jain0Jasmita Dass1Hara Prasad Pati2All India Institute of Medical SciencesAll India Institute of Medical SciencesAll India Institute of Medical SciencesHigh performance liquid chromatography (HPLC) and electrophoresis are commonly used to diagnose various hemoglobinopathies. However, insufficient information about the transfusion history can lead to unexpected and confusing results. We are reporting a case of Juvenile myelomonocytic leukemia (JMML) in which HbHPLC was done to quantify fetal hemoglobin (HbF). The chromatogram showed elevated HbF along with a peak in the HbD window. A transfusion acquired peak was suspected based on the unexpectedly low percentage of HbD and was subsequently confirmed using parental HbHPLC.http://www.mjhid.org/index.php/mjhid/article/view/382HPLC, Transfusion, HbD
collection DOAJ
language English
format Article
sources DOAJ
author Sonal Jain
Jasmita Dass
Hara Prasad Pati
spellingShingle Sonal Jain
Jasmita Dass
Hara Prasad Pati
Transfusion associated peak in Hb HPLC chromatogram – a case report
Mediterranean Journal of Hematology and Infectious Diseases
HPLC, Transfusion, HbD
author_facet Sonal Jain
Jasmita Dass
Hara Prasad Pati
author_sort Sonal Jain
title Transfusion associated peak in Hb HPLC chromatogram – a case report
title_short Transfusion associated peak in Hb HPLC chromatogram – a case report
title_full Transfusion associated peak in Hb HPLC chromatogram – a case report
title_fullStr Transfusion associated peak in Hb HPLC chromatogram – a case report
title_full_unstemmed Transfusion associated peak in Hb HPLC chromatogram – a case report
title_sort transfusion associated peak in hb hplc chromatogram – a case report
publisher PAGEPress Publications
series Mediterranean Journal of Hematology and Infectious Diseases
issn 2035-3006
publishDate 2012-01-01
description High performance liquid chromatography (HPLC) and electrophoresis are commonly used to diagnose various hemoglobinopathies. However, insufficient information about the transfusion history can lead to unexpected and confusing results. We are reporting a case of Juvenile myelomonocytic leukemia (JMML) in which HbHPLC was done to quantify fetal hemoglobin (HbF). The chromatogram showed elevated HbF along with a peak in the HbD window. A transfusion acquired peak was suspected based on the unexpectedly low percentage of HbD and was subsequently confirmed using parental HbHPLC.
topic HPLC, Transfusion, HbD
url http://www.mjhid.org/index.php/mjhid/article/view/382
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AT jasmitadass transfusionassociatedpeakinhbhplcchromatogramacasereport
AT haraprasadpati transfusionassociatedpeakinhbhplcchromatogramacasereport
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