| Summary: | This research was the first karyotypic study of Small-toothed palm civet (Arctogalidia trivirgata). Blood samples were taken from two males and two females kept in Dusit Zoo, Bangkok. After the standard whole blood lymphocyte culture at 37ºC for 72 hr. in presence of Colchicine, the metaphase spreads were performed on microscopic slides and air-dried. Conventional Giemsa's staining were applied to stain the chromosome. The results showed that the number of diploid chromosome of Small-toothed palm civet was 2n = 40, the fundamental number (NF) was 66 in both male and female. The type of autosomes were 2 large metacentric, 2 large submetacentric, 8 large acrocentric, 4 large telocentric, 4 medium submetacentric, 4 medium telocentric, 4 small metacentric, 2 small submetacentric, 2 small acrocentric and 6 small telocentric chromosomes. In addition, chromosome 17 showed a clearly observable satellite. X-chromosome was the large metacentric and Y chromosome was the smallest acrocentric chromosome.The karyotype formula for the male Small-toothed palm civet (Arctogalidia trivirgata) is as follows:2n (40) = Lm 2+Lsm 2+La 8+Lt 4+Msm 4+Mt 4+Sm 4+Ssm 2+Sa 2+St 6+X+Y = Lm 3+Lsm 2+La 8+Lt 4+Msm 4+Mt 4+Sm 4+Ssm 2+Sa 3+St 6The karyotype formula for the female Small-toothed palm civet (Arctogalidia trivirgata) is as follows:2n (40) = Lm 2+Lsm 2+La 8+Lt 4+Msm 4+Mt 4+Sm 4+Ssm 2+Sa 2+St 6+X+X = Lm 4+Lsm 2+La 8+Lt 4+Msm 4+Mt 4+Sm 4+Ssm 2+Sa 2+St 6
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