In vivo sensitivity reduction of Puccinia triticina races, causal agent of wheat leaf rust, to DMI and QoI fungicides Redução da sensibilidade de raças de Puccinia triticina, agente causal da ferrugem da folha do trigo, aos fungicidas DMI e QoI, in vivo

Experiments were carried out to determine in vivo the IC50 and the IC90 for demethylation-inhibitor fungicides (DMIs, triazoles) and quinone outside inhibitors (QoIs, strobilurins) to the five most frequent races of Puccinia triticina in 2007 growing season in Southern Brazil. The tests were done in...

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Bibliographic Details
Main Authors: Gisele da Silva Arduim, Erlei Melo Reis, Amarilis Labes Barcellos, Camila Turra
Format: Article
Language:English
Published: Grupo Paulista de Fitopatologia 2012-12-01
Series:Summa Phytopathologica
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Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052012000400006
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Summary:Experiments were carried out to determine in vivo the IC50 and the IC90 for demethylation-inhibitor fungicides (DMIs, triazoles) and quinone outside inhibitors (QoIs, strobilurins) to the five most frequent races of Puccinia triticina in 2007 growing season in Southern Brazil. The tests were done in a greenhouse with wheat seedlings. DMI fungicides were tested at the concentrations, in mg/L, 0.0; 0.02; 0.2; 2.0; 20.0; 100.0 and 200.0, and QoIs at the concentrations 0.0; 0.0001; 0.001; 0.01; 0.1; 1 and 10.0 mg of active ingredient/L water. Fungicides were preventively applied at 24 hours before the inoculation of seedlings with the fungal spores. The effect of treatments was assessed based on the number of uredia/cm². The lowest IC50 (inhibitory concentration) for DMI fungicides determined for MCG-MN, sensitive race, ranged from 0.33 to 0.91 mg/L, while the highest values for MDP-MR, MDT-MR, MDK-MR, MFH-HT races, varied from 9.63 to 85.64 mg/L (suspected insensitivity). QoI fungicide presented an IC50 varying from 0.0018 to 0.14 mg/L. The sensitivity reduction factor for DMIs varied from 8.8 to 238.8, and for QoIs from 0.3 to 1.5 mg/L. Sensitivity reduction was confirmed for the races MDP-MR, MDT-MR, MDK-MR, MFH-HT to DMIs, as well as their sensitivity to QoI fungicides.<br>Experimentos foram conduzidos para determinar, in vivo a IC50 e CI90 para fungicidas inibidores da demetilação (IDM)(triazois) e inibidores da quinona externa (IQe) (estrobilurinas) a cinco raças de Puccinia triticina mais frequentes na safra 2007, no Sul do Brasil. Os experimentos foram realizados em câmara de crescimento com plântulas de trigo. Os fungicidas IDMs foram testados nas concentrações em mg/L de 0,0; 0,02; 0,2; 2,0; 20,0; 100,0 e 200,0 e os IQes nas concentrações de 0,0; 0,0001; 0,001; 0,01; 0,1, 1 e 10,0 mg de ingrediente activo/L de água. Os fungicidas foram aplicados preventivamente 24 horas antes da inoculação das plântulas com os esporos do fungo. O efeito dos tratamentos foi avaliado pelo número de urdias/cm². A menor IC50 (concentração inibitória) para fungicidas DMI determinados para MCG-MN, raça sensível, variaram de 0,33 a 0,91 e os maiores valores de MDP-MR, MDT-MR, MDK-MR, MFH-HT corridas, variou de 9,63 a 85,64 mg/L (suspeitas de insensibilidade). A CI50 dos fungicidas IQes variou de 0,0018-0,14 mg/L. O fator de redução da sensibilidade para os DMIs variou de 8,8-238,8 e para os IQEs de 0,3-1,5 mg/L. Foi confirmada a redução na sensibilidade de raças MDP-MR, MDT-MR, MDK-MR, MFH-HT para DMIS e sua sensibilidade aos fungicidas IQe.
ISSN:0100-5405