Radiation effects of nuclear physics rays on hepatoma cells

• Main Authors: Zhao Wenhui, Cong Lu, Guerrero-Sánchez Yolanda
• Format: Article
• Language: English
• Published: De Gruyter 2019-04-01
• Series: Open Physics
• Subjects: γ-ray; x-ray; hepatoma cell; radiation effect; cloning formation method; electrophoresis detection; research; 21.65.+f; 28.41.te; 29.30.kv
• Online Access: https://doi.org/10.1515/phys-2019-0017
• ISSN: 2391-5471

The traditional method of cloning formation is used to study the radiation effect of nuclear physics rays on hepatoma cells, and the results obtained are not reliable. Therefore, a new method of studying the radiation effect of nuclear physics rays on hepatoma cells is proposed. PCC method is used to study the radiation effect of γ-rays on hepatoma cells. Radiation effects of X-rays on hepatoma cells were studied by radionuclide formation assay, neutral comet electrophoresis and γH2AX focal detection. The results showed that the survival curves of HepG2 hepatoma cells irradiated by γ-rays were well fitted by linear squares. There was a linear relationship between the survival rate and irradiation dose of HepG2 hepatoma cells irradiated by γ-rays. HepG2 hepatoma cells showed strong tolerance to X-ray irradiation, and the positive rate of γH2AX cells reached 100% in each dose group only 0.5 h after X-ray irradiation.