Effectiveness of calcium chloride in reduction of shoot necrosis incocoa (Theobroma cacao L.) in vitro propagation

Various efforts have been developed for the optimization of the various stages in vitro micropropagation. The maturation stage and pre-acclimatization plantlets is an important stages that must be considered to produce vigorous plants and ready to be planted in the field. The purpose of this study...

Full description

Bibliographic Details
Main Author: Sulistyani Pancaningtyas
Format: Article
Language:English
Published: Indonesian Coffee and Cocoa Research Institute 2012-05-01
Series:Coffee and Cocoa Research Journal
Online Access:http://www.ccrjournal.com/index.php/ccrj/article/view/161
id doaj-8b28053046c24bb2830bf63fba443411
record_format Article
spelling doaj-8b28053046c24bb2830bf63fba4434112020-11-25T03:00:54ZengIndonesian Coffee and Cocoa Research InstituteCoffee and Cocoa Research Journal0215-02122406-95742012-05-0128110.22302/iccri.jur.pelitaperkebunan.v28i1.161149Effectiveness of calcium chloride in reduction of shoot necrosis incocoa (Theobroma cacao L.) in vitro propagationSulistyani Pancaningtyas0Indonesian Coffee and Cocoa Research Institute Various efforts have been developed for the optimization of the various stages in vitro micropropagation. The maturation stage and pre-acclimatization plantlets is an important stages that must be considered to produce vigorous plants and ready to be planted in the field. The purpose of this study was to reduced shoot tip necrosis in cocoa planlets to obtaine vigorous planlets from in vitro propagation through the addition of Calcium Chloride (CaCl2). The study used two stages of embryogenic development. The first was embryo maturation stage and the second was the shoot growth development stage. The study was arranged factorially in experiment design of Completely Randomized Design consisting of two factors i.e. concentration of CaCl2 consisted of 0, 50, 100, 150, and 200 mg/l and clones consisted of Sulawesi 1 and Sca 6. Each experiment was repeated three times, so the number of combination trials were 5x2x3=30 experimental units. The parameters observed included shoot growth percentage and vigorous planlets percentage. The results showed that the addition of CaCl2 at a concentration of 150 mg/l during maturition stage increased the embryos performance and percentage of shoot tip. However, it could not prevent the shoot tip necrosis. Whereas, the addition at a concentration of 50 mg/l during the shoot m growth development stage could reduced necrosis, suggested to increase the quality of in vitro planlets. Key words: Cocoa, necrosis, calcium chloride, somatic embryogenesis, embryos, planlets, in vitro. http://www.ccrjournal.com/index.php/ccrj/article/view/161
collection DOAJ
language English
format Article
sources DOAJ
author Sulistyani Pancaningtyas
spellingShingle Sulistyani Pancaningtyas
Effectiveness of calcium chloride in reduction of shoot necrosis incocoa (Theobroma cacao L.) in vitro propagation
Coffee and Cocoa Research Journal
author_facet Sulistyani Pancaningtyas
author_sort Sulistyani Pancaningtyas
title Effectiveness of calcium chloride in reduction of shoot necrosis incocoa (Theobroma cacao L.) in vitro propagation
title_short Effectiveness of calcium chloride in reduction of shoot necrosis incocoa (Theobroma cacao L.) in vitro propagation
title_full Effectiveness of calcium chloride in reduction of shoot necrosis incocoa (Theobroma cacao L.) in vitro propagation
title_fullStr Effectiveness of calcium chloride in reduction of shoot necrosis incocoa (Theobroma cacao L.) in vitro propagation
title_full_unstemmed Effectiveness of calcium chloride in reduction of shoot necrosis incocoa (Theobroma cacao L.) in vitro propagation
title_sort effectiveness of calcium chloride in reduction of shoot necrosis incocoa (theobroma cacao l.) in vitro propagation
publisher Indonesian Coffee and Cocoa Research Institute
series Coffee and Cocoa Research Journal
issn 0215-0212
2406-9574
publishDate 2012-05-01
description Various efforts have been developed for the optimization of the various stages in vitro micropropagation. The maturation stage and pre-acclimatization plantlets is an important stages that must be considered to produce vigorous plants and ready to be planted in the field. The purpose of this study was to reduced shoot tip necrosis in cocoa planlets to obtaine vigorous planlets from in vitro propagation through the addition of Calcium Chloride (CaCl2). The study used two stages of embryogenic development. The first was embryo maturation stage and the second was the shoot growth development stage. The study was arranged factorially in experiment design of Completely Randomized Design consisting of two factors i.e. concentration of CaCl2 consisted of 0, 50, 100, 150, and 200 mg/l and clones consisted of Sulawesi 1 and Sca 6. Each experiment was repeated three times, so the number of combination trials were 5x2x3=30 experimental units. The parameters observed included shoot growth percentage and vigorous planlets percentage. The results showed that the addition of CaCl2 at a concentration of 150 mg/l during maturition stage increased the embryos performance and percentage of shoot tip. However, it could not prevent the shoot tip necrosis. Whereas, the addition at a concentration of 50 mg/l during the shoot m growth development stage could reduced necrosis, suggested to increase the quality of in vitro planlets. Key words: Cocoa, necrosis, calcium chloride, somatic embryogenesis, embryos, planlets, in vitro.
url http://www.ccrjournal.com/index.php/ccrj/article/view/161
work_keys_str_mv AT sulistyanipancaningtyas effectivenessofcalciumchlorideinreductionofshootnecrosisincocoatheobromacacaolinvitropropagation
_version_ 1724696157969448960