Knockdown of SLC35F2 Inhibits the Proliferation and Metastasis of Bladder Cancer Cells

• Main Authors: Chen M, Gao X, Huang D, Wang S, Zheng L, Chen Y, Wen X, Gao Y, Cao H, Zhang S
• Format: Article
• Language: English
• Published: Dove Medical Press 2019-12-01
• Series: OncoTargets and Therapy
• Subjects: bladder cancer; slc35f2; proliferation; migration; invasion
• Online Access: https://www.dovepress.com/knockdown-of-slc35f2-inhibits-the-proliferation-and-metastasis-of-blad-peer-reviewed-article-OTT

Mei Chen,1 Xin Gao,2 Denggao Huang,1 Shunlan Wang,1 Linlin Zheng,1 Yinyi Chen,3 Xiaohong Wen,1 Yuanhui Gao,1 Hui Cao,1 Shufang Zhang1 1Central Laboratory, Affiliated Haikou Hospital of Xiangya Medical College, Central South University, Haikou 570208, People’s Republic of China; 2Clinical Laboratory, The First People’s Hospital of Huaihua, Huaihua 418000, People’s Republic of China; 3Clinical Laboratory, The First Affiliated Hospital of University of South China, Hengyang 421001, People’s Republic of ChinaCorrespondence: Shufang ZhangCentral Laboratory, Affiliated Haikou Hospital of Xiangya Medical College, Central South University, No.43 Renmin Avenue, Haikou City, Haikou Province 570208, People’s Republic of ChinaEmail [e-mail]: Many studies have shown that solute carrier family 35 member F2 (SLC35F2) plays a key role in the biological processes of multiple cancers. However, there have been no reports on the role of SLC35F2 in the occurrence and development of bladder cancer (BC).Methods: SLC35F2 expression data and clinical and prognostic information from BC patients were obtained from databases. SLC35F2 expression in BC was verified by quantitative real-time PCR (qRT-PCR). The influence of SLC35F2 knockdown on the proliferation, apoptosis, migration and invasion in the 5637 and T24 cell lines was studied, and tumor formation experiments were performed in nude mice. Gene set enrichment analysis (GSEA) was used to predict the pathways and functions of SLC35F2 in BC.Results: SLC35F2 was highly expressed in BC tissues and was associated with invasiveness and T stage in BC patients. SLC35F2 knockdown can inhibit the proliferation, migration and invasion of BC cells and can promote apoptosis. SLC35F2 knockdown significantly reduced tumorigenesis in nude mice. GSEA showed that BC, pathways in cancer, apoptosis and the P53 signaling pathway were significantly enriched in SLC35F2 high expression phenotype.Conclusion: SLC35F2 can promote malignant progression and is a potential therapeutic target in BC.Keywords: bladder cancer, SLC35F2, proliferation, migration, invasion