Development of a system for efficient callus production, somatic embryogenesis and gene editing using CRISPR/Cas9 in Saffron (Crocus sativus L.)

Development of a system for efficient callus production, somatic embryogenesis and gene editing using CRISPR/Cas9 in Saffron (Crocus sativus L.)

Abstract Background Crocus sativus is a recalcitrant plant for genetic transformation and genetic improvement, largely due to difficulties in Agrobacterium mediated transformation and vegetative reproduction. Effective genome editing requires proficient callus production and an efficient method to d...

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Bibliographic Details
Main Authors: Sudha Chib, Arulprakash Thangaraj, Sanjana Kaul, Manoj Kumar Dhar, Tanushri Kaul
Format: Article
Language:English
Published: BMC 2020-04-01
Series:Plant Methods
Subjects:
Cas9
CRISPR
Gene manipulation
Saffron
Invitro regeneration
Transformation
Online Access:http://link.springer.com/article/10.1186/s13007-020-00589-2

Internet

http://link.springer.com/article/10.1186/s13007-020-00589-2

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