Fine-Needle Aspiration-Based Patient-Derived Cancer Organoids
Summary: Patient-derived cancer organoids hold great potential to accurately model and predict therapeutic responses. Efficient organoid isolation methods that minimize post-collection manipulation of tissues would improve adaptability, accuracy, and applicability to both experimental and real-time...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-08-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004220305988 |
| id |
doaj-8afed6fb0a4e4ebb9491411bebb640fc |
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| record_format |
Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Anna E. Vilgelm Kensey Bergdorf Melissa Wolf Vijaya Bharti Rebecca Shattuck-Brandt Ashlyn Blevins Caroline Jones Courtney Phifer Mason Lee Cindy Lowe Rachel Hongo Kelli Boyd James Netterville Sarah Rohde Kamran Idrees Joshua A. Bauer David Westover Bradley Reinfeld Naira Baregamian Ann Richmond W. Kimryn Rathmell Ethan Lee Oliver G. McDonald Vivian L. Weiss |
| spellingShingle |
Anna E. Vilgelm Kensey Bergdorf Melissa Wolf Vijaya Bharti Rebecca Shattuck-Brandt Ashlyn Blevins Caroline Jones Courtney Phifer Mason Lee Cindy Lowe Rachel Hongo Kelli Boyd James Netterville Sarah Rohde Kamran Idrees Joshua A. Bauer David Westover Bradley Reinfeld Naira Baregamian Ann Richmond W. Kimryn Rathmell Ethan Lee Oliver G. McDonald Vivian L. Weiss Fine-Needle Aspiration-Based Patient-Derived Cancer Organoids iScience Clinical Medicine Tissue Engineering Cancer |
| author_facet |
Anna E. Vilgelm Kensey Bergdorf Melissa Wolf Vijaya Bharti Rebecca Shattuck-Brandt Ashlyn Blevins Caroline Jones Courtney Phifer Mason Lee Cindy Lowe Rachel Hongo Kelli Boyd James Netterville Sarah Rohde Kamran Idrees Joshua A. Bauer David Westover Bradley Reinfeld Naira Baregamian Ann Richmond W. Kimryn Rathmell Ethan Lee Oliver G. McDonald Vivian L. Weiss |
| author_sort |
Anna E. Vilgelm |
| title |
Fine-Needle Aspiration-Based Patient-Derived Cancer Organoids |
| title_short |
Fine-Needle Aspiration-Based Patient-Derived Cancer Organoids |
| title_full |
Fine-Needle Aspiration-Based Patient-Derived Cancer Organoids |
| title_fullStr |
Fine-Needle Aspiration-Based Patient-Derived Cancer Organoids |
| title_full_unstemmed |
Fine-Needle Aspiration-Based Patient-Derived Cancer Organoids |
| title_sort |
fine-needle aspiration-based patient-derived cancer organoids |
| publisher |
Elsevier |
| series |
iScience |
| issn |
2589-0042 |
| publishDate |
2020-08-01 |
| description |
Summary: Patient-derived cancer organoids hold great potential to accurately model and predict therapeutic responses. Efficient organoid isolation methods that minimize post-collection manipulation of tissues would improve adaptability, accuracy, and applicability to both experimental and real-time clinical settings. Here we present a simple and minimally invasive fine-needle aspiration (FNA)-based organoid culture technique using a variety of tumor types including gastrointestinal, thyroid, melanoma, and kidney. This method isolates organoids directly from patients at the bedside or from resected tissues, requiring minimal tissue processing while preserving the histologic growth patterns and infiltrating immune cells. Finally, we illustrate diverse downstream applications of this technique including in vitro high-throughput chemotherapeutic screens, in situ immune cell characterization, and in vivo patient-derived xenografts. Thus, routine clinical FNA-based collection techniques represent an unappreciated substantial source of material that can be exploited to generate tumor organoids from a variety of tumor types for both discovery and clinical applications. |
| topic |
Clinical Medicine Tissue Engineering Cancer |
| url |
http://www.sciencedirect.com/science/article/pii/S2589004220305988 |
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doaj-8afed6fb0a4e4ebb9491411bebb640fc2020-11-25T03:20:59ZengElsevieriScience2589-00422020-08-01238101408Fine-Needle Aspiration-Based Patient-Derived Cancer OrganoidsAnna E. Vilgelm0Kensey Bergdorf1Melissa Wolf2Vijaya Bharti3Rebecca Shattuck-Brandt4Ashlyn Blevins5Caroline Jones6Courtney Phifer7Mason Lee8Cindy Lowe9Rachel Hongo10Kelli Boyd11James Netterville12Sarah Rohde13Kamran Idrees14Joshua A. Bauer15David Westover16Bradley Reinfeld17Naira Baregamian18Ann Richmond19W. Kimryn Rathmell20Ethan Lee21Oliver G. McDonald22Vivian L. Weiss23Department of Pathology, The Ohio State University, Columbus, OH 43210, USADepartment of Pharmacology, Vanderbilt University, Nashville, TN 37232, USADepartment of Biochemistry, Vanderbilt University, Nashville, TN 37232, USADepartment of Pathology, The Ohio State University, Columbus, OH 43210, USA; Department of Pharmacology, Vanderbilt University, Nashville, TN 37232, USADepartment of Pharmacology, Vanderbilt University, Nashville, TN 37232, USADepartment of Pharmacology, Vanderbilt University, Nashville, TN 37232, USADepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USADepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USADepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USADepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USADepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USADepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Vanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN 37232, USAVanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Department of Surgery, Vanderbilt University Medical Center, Nashville, TN 37232, USAVanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Department of Surgery, Vanderbilt University Medical Center, Nashville, TN 37232, USAVanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Department of Surgery, Vanderbilt University Medical Center, Nashville, TN 37232, USADepartment of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA; Vanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Vanderbilt Institute of Chemical Biology - High-Throughput Screening Facility, Vanderbilt University, Nashville, TN 37232, USADepartment of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA; Vanderbilt Institute of Chemical Biology - High-Throughput Screening Facility, Vanderbilt University, Nashville, TN 37232, USADepartment of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USAVanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Department of Surgery, Vanderbilt University Medical Center, Nashville, TN 37232, USADepartment of Pharmacology, Vanderbilt University, Nashville, TN 37232, USA; Vanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN 37232, USAVanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USADepartment of Pharmacology, Vanderbilt University, Nashville, TN 37232, USA; Vanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN 37232, USADepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Vanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Epithelial Biology Center, Vanderbilt University Medical Center, Nashville, TN 37232, USADepartment of Pharmacology, Vanderbilt University, Nashville, TN 37232, USA; Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Vanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Corresponding authorSummary: Patient-derived cancer organoids hold great potential to accurately model and predict therapeutic responses. Efficient organoid isolation methods that minimize post-collection manipulation of tissues would improve adaptability, accuracy, and applicability to both experimental and real-time clinical settings. Here we present a simple and minimally invasive fine-needle aspiration (FNA)-based organoid culture technique using a variety of tumor types including gastrointestinal, thyroid, melanoma, and kidney. This method isolates organoids directly from patients at the bedside or from resected tissues, requiring minimal tissue processing while preserving the histologic growth patterns and infiltrating immune cells. Finally, we illustrate diverse downstream applications of this technique including in vitro high-throughput chemotherapeutic screens, in situ immune cell characterization, and in vivo patient-derived xenografts. Thus, routine clinical FNA-based collection techniques represent an unappreciated substantial source of material that can be exploited to generate tumor organoids from a variety of tumor types for both discovery and clinical applications.http://www.sciencedirect.com/science/article/pii/S2589004220305988Clinical MedicineTissue EngineeringCancer |