Preliminary Study: Purple Sweet Potato Extract Seems to Be Superior to Increase the Migration of Impaired Endothelial Progenitor Cells Compared to <span style="font-variant: small-caps">l</span>-Ascorbic Acid

• Main Authors: Yudi Her Oktaviono, Makhyan Jibril Al-Farabi, Luh Oliva Saraswati Suastika, Febriyanti Hartono, Yanni Dirgantara, Ferry Sandra
• Format: Article
• Language: English
• Published: Österreichische Apotheker-Verlagsgesellschaft m. b. H. 2019-07-01
• Series: Scientia Pharmaceutica
• Subjects: antioxidant; anthocyanin; coronary heart disease; functional
• Online Access: https://www.mdpi.com/2218-0532/87/3/16

Impairment of the endothelial progenitor cells (EPCs) ability to proliferate and migrate in the patients with coronary heart disease (CHD) is partly caused by oxidative stress. This research evaluates the effect of treatment with <i>Ipomoea batatas</i> L./purple sweet potato (PSP) extract and <span style="font-variant: small-caps;">l</span>-ascorbic acid on the proliferation and migration of impaired EPCs. EPCs were isolated from CHD patient&#8217;s peripheral blood. EPCs culture were cultivated and divided into control (untreated), PSP extract treatment (dose 1 and 25 &#956;g/mL), and <span style="font-variant: small-caps;">l</span>-ascorbic acid treatment (dose 10 and 250 &#956;g/mL) groups for 48 h. EPCs proliferation was analyzed with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay, and migration was evaluated with the cell migration assay kit. Statistical tests were evaluated using SPSS 25.0. This research showed that EPCs proliferation and migration was significantly higher in all PSP extract and <span style="font-variant: small-caps;">l</span>-ascorbic acid treatment compared to the control (<i>p</i> &lt; 0.001). EPCs migration on treatment with a PSP extract dose of 25 &#956;g/mL was significantly higher compared to the treatment with <span style="font-variant: small-caps;">l</span>-ascorbic acid dose of 250 &#956;g/mL (303,000 &#177; 1000 compared to 215,000 &#177; 3000 cells, <i>p&lt;</i> 0.001). In conclusion, both treatments with PSP extract and <span style="font-variant: small-caps;">l</span>-ascorbic acid can improve the proliferation and migration of impaired EPCs. At the dose of 25 &#956;g/mL, PSP extract seems to be superior to the <span style="font-variant: small-caps;">l</span>-ascorbic acid dose of 250 &#956;g/mL to improve EPCs migration.