Specular Microscopic Features of Corneal Endothelial Vacuolation

• Main Authors: Mozhgan Rezaei Kanavi, Mohammad-Ali Javadi, Tahereh Chamani
• Format: Article
• Language: English
• Published: Knowledge E 2011-01-01
• Series: Journal of Ophthalmic & Vision Research
• Subjects: Specular Microscopy; Cornea; Endothelium; Vacuole
• Online Access: http://www.jovr.org/article.asp?issn=2008-322X;year=2011;volume=6;issue=1;spage=5;epage=7;aulast=Kanavi

Purpose: To introduce a specular microscopic reference image for endothelial vacuolation in donated corneas. Methods: Two corneas from a donor with diffuse, round to oval dark areas at the endothelial level on slit lamp biomicroscopy and one normal-appearing donor cornea underwent specular microscopy, histopathologic evaluation and transmission electron microscopy. Results: Specular microscopy of the two corneas with abnormal-looking endothelium revealed large numbers of dark, round to oval structures within the endothelium in favor of endothelial vacuolation. Light microscopy disclosed variable sized cyst-like structures within the cytoplasm. Transmission electron microscopy showed electronlucent and relatively large-sized intracytoplasmic vacuoles. These features were not observed in the endothelium of the normal cornea. Conclusion: The specular microscopic features of endothelial vacuolation in donated corneas were confirmed by light microscopy and transmission electron microscopy, therefore the specular image may be proposed as a reference to eye banks.