Photobleaching Imprinting Enhanced Background Rejection in Line-Scanning Temporal Focusing Microscopy

Compared with two-photon point-scanning microscopy, two-photon temporal focusing microscopy (2pTFM) provides a parallel high-speed imaging strategy with optical sectioning capability. Owing to out-of-focus fluorescence induced by scattering, 2pTFM suffers deteriorated signal-to-background ratio (SBR...

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Bibliographic Details
Main Authors: Chaowei Zhuang, Xinyang Li, Yuanlong Zhang, Lingjie Kong, Hao Xie, Qionghai Dai
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-12-01
Series:Frontiers in Chemistry
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fchem.2020.618131/full