Effect of ferri(III)citrate and potassium hexacyanoferrate(III) on growth of the moss Bryum argenteum Hedw. (Bryaceae) in vitro

• Main Authors: Vukojević Vanja, Sabovljević Aneta, Sabovljević Marko S.
• Format: Article
• Language: English
• Published: University of Belgrade, University of Novi Sad 2004-01-01
• Series: Archives of Biological Sciences
• Online Access: http://www.doiserbia.nb.rs/img/doi/0354-4664/2004/0354-46640404075V.pdf

In order to examine the manner of iron uptake from the medium, in vitro culture of moss Bryum argenteum Hedw. (Bryaceae) was established. Under controlled conditions (16h light/8h dark, light intensity 47μmol m-2s-1 25±2°C), the moss was grown on basal MS medium or on MS medium enriched with various concentrations of ferri(III)citrate or potassium hexacyanoferrate(III). It was expected that with the organic chelate complex, Fe(III) ion will be more available for the plant. Sixty days after establishing in vitro culture, the plants grown on MS medium enriched with the ferri(III)citrate complex were developed better than plants grown on media with the potassium hexacyanoferrate(III) complex. To judge from plant production in vitro and in view of the fact that the two compounds were the only source of Fe(III), it would appear that the citrate complex makes Fe(III) ions more available than potassium hexacyanoferrate(III). Further research will examine the concentrations of Fe ion uptake by plants and potential use of these tiny moss plants for the phytomining, phytoremedies and hyperaccumulating purposes.