Cardioprotective Effect of Saffron Extract and Safranal in Isoproterenol-Induced Myocardial Infarction in Wistar Rats

• Main Authors: Roya Mehdizadeh, Mohammad–Reza Parizadeh, Ali-Reza Khooei, Soghra Mehri, Hossein Hosseinzadeh
• Format: Article
• Language: English
• Published: Mashhad University of Medical Sciences 2013-01-01
• Series: Iranian Journal of Basic Medical Sciences
• Subjects: Crocus sativus; Isoproterenol; Lipid peroxidation; Myocardial infarction; oxidative stress; saffron; Safranal
• Online Access: http://ijbms.mums.ac.ir/pdf_249_cbaa2449e214d3ee72e62f117afe4402.html

Objective(s):This study was designed to evaluate the cardioprotective effect of Crocus sativus L. (saffron)aqueous extract and safranal, the major constituent of the essential oil of saffron, on lipid peroxidation, biochemical parameters and histopathological findings in isoproterenol (ISO)-induced myocardial infarction in Wistar rats. Materials and Methods:The saffron extract (20, 40, 80 and 160 mg/kg/day IP) or control were administered for 9 days along with ISO (85 mg/kg, SC, at 24 hr interval) on 8th and 9th day in rats. Activities of creatine kinase-muscle, brain (CK-MB) and lactate dehydrogenase (LDH) were measured using standard commercial kits. The level of malondialdehyde in heart tissue was estimated with thiobarbituric acid reactive species test. For histopathological examination, hematoxylin and eosin (H&E) staining was used. Results:ISO administration induced a statistically significant increase          (P