Butyrate Permeation across the Isolated Ovine Reticulum Epithelium

• Main Authors: Reiko Rackwitz, Franziska Dengler, Gotthold Gäbel
• Format: Article
• Language: English
• Published: MDPI AG 2020-11-01
• Series: Animals
• Subjects: short-chain fatty acids (SCFA); transepithelial transport; MCT1; NHE; Na⁺/K⁺-ATPase; reticulorumen
• Online Access: https://www.mdpi.com/2076-2615/10/12/2198

We hypothesized that, due to the high pH of this compartment, the reticulum epithelium displays particular features in the transport of short-chain fatty acids (SCFA). Ovine reticulum epithelium was incubated in Ussing chambers using a bicarbonate-free buffer solution containing butyrate (20 mmol L⁻¹). p-hydroxymercuribenzoic acid (pHMB), 5-(N-Ethyl-N-isopropyl)amiloride (EIPA), or ouabain were added to the buffer solution as inhibitors of monocarboxylate transporters, sodium-proton-exchangers, or the Na⁺/K⁺-ATPase, respectively. The short-circuit current (I_sc) and transepithelial conductance (G_t) were monitored continuously while the flux rates of ¹⁴C-labelled butyrate were measured in the mucosal-to-serosal (J_ms^but) or serosal-to-mucosal direction (J_sm^but). Under control conditions, the mean values of I_sc and G_t amounted to 2.54 ± 0.46 µEq cm⁻² h⁻¹ and 6.02 ± 3.3 mS cm⁻², respectively. J_ms^but was 2.1 ± 1.01 µmol cm⁻² h⁻¹ on average and about twice as high as J_sm^but. Incubation with ouabain reduced J_ms^but, while J_sm^but was not affected. The serosal addition of EIPA did not affect J_ms^but but reduced J_sm^but by about 10%. The addition of pHMB to the mucosal or serosal solution reduced J_ms^but but had no effect on J_sm^but. Mucosally applied pHMB provoked a transient increase in the I_sc. The serosal pHMB sharply reduced I_sc. Our results demonstrate that butyrate can be effectively transported across the reticulum epithelium. The mechanisms involved in this absorption differ from those known from the rumen epithelium.