Overexpression of Pear (<i>Pyrus pyrifolia</i>) <i>CAD2</i> in Tomato Affects Lignin Content

Overexpression of Pear (<i>Pyrus pyrifolia</i>) <i>CAD2</i> in Tomato Affects Lignin Content

<i>PpCAD2</i> was originally isolated from the &#8216;Wangkumbae&#8217; pear (<i>Pyrus pyrifolia</i> Nakai), and it encodes for cinnamyl alcohol dehydrogenase (CAD), which is a key enzyme in the lignin biosynthesis pathway. In order to verify the function of <i>...

Full description

Bibliographic Details
Main Authors: Mingtong Li, Chenxia Cheng, Xinfu Zhang, Suping Zhou, Lixia Li, Shaolan Yang
Format: Article
Language:English
Published: MDPI AG 2019-07-01
Series:Molecules
Subjects:
<i>PpCAD2</i>
transgenic tomato
lignin
Online Access:https://www.mdpi.com/1420-3049/24/14/2595
id doaj-89c42a98268642628f1f84916be4dd7a
record_format Article
spelling doaj-89c42a98268642628f1f84916be4dd7a2020-11-24T21:55:22ZengMDPI AGMolecules1420-30492019-07-012414259510.3390/molecules24142595molecules24142595Overexpression of Pear (<i>Pyrus pyrifolia</i>) <i>CAD2</i> in Tomato Affects Lignin ContentMingtong Li0Chenxia Cheng1Xinfu Zhang2Suping Zhou3Lixia Li4Shaolan Yang5College of Horticulture, Qingdao Agricultural University, 700 Changcheng Road, Chengyang, Qingdao City 266109, ChinaCollege of Horticulture, Qingdao Agricultural University, 700 Changcheng Road, Chengyang, Qingdao City 266109, ChinaCollege of Horticulture, Qingdao Agricultural University, 700 Changcheng Road, Chengyang, Qingdao City 266109, ChinaDepartment of Agricultural and Environmental Sciences, College of Agriculture, Tennessee State University, 3500 John Merritt Blvd, Nashville, TN 37209, USADongying Academy of Agricultural Science, Dongying 257091, ChinaCollege of Horticulture, Qingdao Agricultural University, 700 Changcheng Road, Chengyang, Qingdao City 266109, China<i>PpCAD2</i> was originally isolated from the &#8216;Wangkumbae&#8217; pear (<i>Pyrus pyrifolia</i> Nakai), and it encodes for cinnamyl alcohol dehydrogenase (CAD), which is a key enzyme in the lignin biosynthesis pathway. In order to verify the function of <i>PpCAD2</i>, transgenic tomato (<i>Solanum lycopersicum</i>) &#8216;Micro-Tom&#8217; plants were generated using over-expression constructs via the agrobacterium-mediated transformation method. The results showed that the <i>PpCAD2</i> over-expression transgenic tomato plant had a strong growth vigor. Furthermore, these <i>PpCAD2</i> over-expression transgenic tomato plants contained a higher lignin content and CAD enzymatic activity in the stem, leaf and fruit pericarp tissues, and formed a greater number of vessel elements in the stem and leaf vein, compared to wild type tomato plants. This study clearly indicated that overexpressing <i>PpCAD2</i> increased the lignin deposition of transgenic tomato plants, and thus validated the function of <i>PpCAD2</i> in lignin biosynthesis.https://www.mdpi.com/1420-3049/24/14/2595<i>PpCAD2</i>transgenic tomatolignin
collection DOAJ
language English
format Article
sources DOAJ
author Mingtong Li
Chenxia Cheng
Xinfu Zhang
Suping Zhou
Lixia Li
Shaolan Yang
spellingShingle Mingtong Li
Chenxia Cheng
Xinfu Zhang
Suping Zhou
Lixia Li
Shaolan Yang
Overexpression of Pear (<i>Pyrus pyrifolia</i>) <i>CAD2</i> in Tomato Affects Lignin Content
Molecules
<i>PpCAD2</i>
transgenic tomato
lignin
author_facet Mingtong Li
Chenxia Cheng
Xinfu Zhang
Suping Zhou
Lixia Li
Shaolan Yang
author_sort Mingtong Li
title Overexpression of Pear (<i>Pyrus pyrifolia</i>) <i>CAD2</i> in Tomato Affects Lignin Content
title_short Overexpression of Pear (<i>Pyrus pyrifolia</i>) <i>CAD2</i> in Tomato Affects Lignin Content
title_full Overexpression of Pear (<i>Pyrus pyrifolia</i>) <i>CAD2</i> in Tomato Affects Lignin Content
title_fullStr Overexpression of Pear (<i>Pyrus pyrifolia</i>) <i>CAD2</i> in Tomato Affects Lignin Content
title_full_unstemmed Overexpression of Pear (<i>Pyrus pyrifolia</i>) <i>CAD2</i> in Tomato Affects Lignin Content
title_sort overexpression of pear (<i>pyrus pyrifolia</i>) <i>cad2</i> in tomato affects lignin content
publisher MDPI AG
series Molecules
issn 1420-3049
publishDate 2019-07-01
description <i>PpCAD2</i> was originally isolated from the &#8216;Wangkumbae&#8217; pear (<i>Pyrus pyrifolia</i> Nakai), and it encodes for cinnamyl alcohol dehydrogenase (CAD), which is a key enzyme in the lignin biosynthesis pathway. In order to verify the function of <i>PpCAD2</i>, transgenic tomato (<i>Solanum lycopersicum</i>) &#8216;Micro-Tom&#8217; plants were generated using over-expression constructs via the agrobacterium-mediated transformation method. The results showed that the <i>PpCAD2</i> over-expression transgenic tomato plant had a strong growth vigor. Furthermore, these <i>PpCAD2</i> over-expression transgenic tomato plants contained a higher lignin content and CAD enzymatic activity in the stem, leaf and fruit pericarp tissues, and formed a greater number of vessel elements in the stem and leaf vein, compared to wild type tomato plants. This study clearly indicated that overexpressing <i>PpCAD2</i> increased the lignin deposition of transgenic tomato plants, and thus validated the function of <i>PpCAD2</i> in lignin biosynthesis.
topic <i>PpCAD2</i>
transgenic tomato
lignin
url https://www.mdpi.com/1420-3049/24/14/2595
work_keys_str_mv AT mingtongli overexpressionofpearipyruspyrifoliaiicad2iintomatoaffectslignincontent
AT chenxiacheng overexpressionofpearipyruspyrifoliaiicad2iintomatoaffectslignincontent
AT xinfuzhang overexpressionofpearipyruspyrifoliaiicad2iintomatoaffectslignincontent
AT supingzhou overexpressionofpearipyruspyrifoliaiicad2iintomatoaffectslignincontent
AT lixiali overexpressionofpearipyruspyrifoliaiicad2iintomatoaffectslignincontent
AT shaolanyang overexpressionofpearipyruspyrifoliaiicad2iintomatoaffectslignincontent
_version_ 1725863099524710400

Similar Items