Uropathogenic E. coli induces DNA damage in the bladder.

Urinary tract infections (UTIs) are among the most common outpatient infections, with a lifetime incidence of around 60% in women. We analysed urine samples from 223 patients with community-acquired UTIs and report the presence of the cleavage product released during the synthesis of colibactin, a b...

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Main Authors: Camille V Chagneau, Clémence Massip, Nadège Bossuet-Greif, Christophe Fremez, Jean-Paul Motta, Ayaka Shima, Céline Besson, Pauline Le Faouder, Nicolas Cénac, Marie-Paule Roth, Hélène Coppin, Maxime Fontanié, Patricia Martin, Jean-Philippe Nougayrède, Eric Oswald
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2021-02-01
Series:PLoS Pathogens
Online Access:https://doi.org/10.1371/journal.ppat.1009310
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spelling doaj-89b242f36991425a8d871d48eba73d412021-06-26T04:31:00ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742021-02-01172e100931010.1371/journal.ppat.1009310Uropathogenic E. coli induces DNA damage in the bladder.Camille V ChagneauClémence MassipNadège Bossuet-GreifChristophe FremezJean-Paul MottaAyaka ShimaCéline BessonPauline Le FaouderNicolas CénacMarie-Paule RothHélène CoppinMaxime FontaniéPatricia MartinJean-Philippe NougayrèdeEric OswaldUrinary tract infections (UTIs) are among the most common outpatient infections, with a lifetime incidence of around 60% in women. We analysed urine samples from 223 patients with community-acquired UTIs and report the presence of the cleavage product released during the synthesis of colibactin, a bacterial genotoxin, in 55 of the samples examined. Uropathogenic Escherichia coli strains isolated from these patients, as well as the archetypal E. coli strain UTI89, were found to produce colibactin. In a murine model of UTI, the machinery producing colibactin was expressed during the early hours of the infection, when intracellular bacterial communities form. We observed extensive DNA damage both in umbrella and bladder progenitor cells. To the best of our knowledge this is the first report of colibactin production in UTIs in humans and its genotoxicity in bladder cells.https://doi.org/10.1371/journal.ppat.1009310
collection DOAJ
language English
format Article
sources DOAJ
author Camille V Chagneau
Clémence Massip
Nadège Bossuet-Greif
Christophe Fremez
Jean-Paul Motta
Ayaka Shima
Céline Besson
Pauline Le Faouder
Nicolas Cénac
Marie-Paule Roth
Hélène Coppin
Maxime Fontanié
Patricia Martin
Jean-Philippe Nougayrède
Eric Oswald
spellingShingle Camille V Chagneau
Clémence Massip
Nadège Bossuet-Greif
Christophe Fremez
Jean-Paul Motta
Ayaka Shima
Céline Besson
Pauline Le Faouder
Nicolas Cénac
Marie-Paule Roth
Hélène Coppin
Maxime Fontanié
Patricia Martin
Jean-Philippe Nougayrède
Eric Oswald
Uropathogenic E. coli induces DNA damage in the bladder.
PLoS Pathogens
author_facet Camille V Chagneau
Clémence Massip
Nadège Bossuet-Greif
Christophe Fremez
Jean-Paul Motta
Ayaka Shima
Céline Besson
Pauline Le Faouder
Nicolas Cénac
Marie-Paule Roth
Hélène Coppin
Maxime Fontanié
Patricia Martin
Jean-Philippe Nougayrède
Eric Oswald
author_sort Camille V Chagneau
title Uropathogenic E. coli induces DNA damage in the bladder.
title_short Uropathogenic E. coli induces DNA damage in the bladder.
title_full Uropathogenic E. coli induces DNA damage in the bladder.
title_fullStr Uropathogenic E. coli induces DNA damage in the bladder.
title_full_unstemmed Uropathogenic E. coli induces DNA damage in the bladder.
title_sort uropathogenic e. coli induces dna damage in the bladder.
publisher Public Library of Science (PLoS)
series PLoS Pathogens
issn 1553-7366
1553-7374
publishDate 2021-02-01
description Urinary tract infections (UTIs) are among the most common outpatient infections, with a lifetime incidence of around 60% in women. We analysed urine samples from 223 patients with community-acquired UTIs and report the presence of the cleavage product released during the synthesis of colibactin, a bacterial genotoxin, in 55 of the samples examined. Uropathogenic Escherichia coli strains isolated from these patients, as well as the archetypal E. coli strain UTI89, were found to produce colibactin. In a murine model of UTI, the machinery producing colibactin was expressed during the early hours of the infection, when intracellular bacterial communities form. We observed extensive DNA damage both in umbrella and bladder progenitor cells. To the best of our knowledge this is the first report of colibactin production in UTIs in humans and its genotoxicity in bladder cells.
url https://doi.org/10.1371/journal.ppat.1009310
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