Uropathogenic E. coli induces DNA damage in the bladder.
Urinary tract infections (UTIs) are among the most common outpatient infections, with a lifetime incidence of around 60% in women. We analysed urine samples from 223 patients with community-acquired UTIs and report the presence of the cleavage product released during the synthesis of colibactin, a b...
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2021-02-01
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Series: | PLoS Pathogens |
Online Access: | https://doi.org/10.1371/journal.ppat.1009310 |
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doaj-89b242f36991425a8d871d48eba73d412021-06-26T04:31:00ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742021-02-01172e100931010.1371/journal.ppat.1009310Uropathogenic E. coli induces DNA damage in the bladder.Camille V ChagneauClémence MassipNadège Bossuet-GreifChristophe FremezJean-Paul MottaAyaka ShimaCéline BessonPauline Le FaouderNicolas CénacMarie-Paule RothHélène CoppinMaxime FontaniéPatricia MartinJean-Philippe NougayrèdeEric OswaldUrinary tract infections (UTIs) are among the most common outpatient infections, with a lifetime incidence of around 60% in women. We analysed urine samples from 223 patients with community-acquired UTIs and report the presence of the cleavage product released during the synthesis of colibactin, a bacterial genotoxin, in 55 of the samples examined. Uropathogenic Escherichia coli strains isolated from these patients, as well as the archetypal E. coli strain UTI89, were found to produce colibactin. In a murine model of UTI, the machinery producing colibactin was expressed during the early hours of the infection, when intracellular bacterial communities form. We observed extensive DNA damage both in umbrella and bladder progenitor cells. To the best of our knowledge this is the first report of colibactin production in UTIs in humans and its genotoxicity in bladder cells.https://doi.org/10.1371/journal.ppat.1009310 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Camille V Chagneau Clémence Massip Nadège Bossuet-Greif Christophe Fremez Jean-Paul Motta Ayaka Shima Céline Besson Pauline Le Faouder Nicolas Cénac Marie-Paule Roth Hélène Coppin Maxime Fontanié Patricia Martin Jean-Philippe Nougayrède Eric Oswald |
spellingShingle |
Camille V Chagneau Clémence Massip Nadège Bossuet-Greif Christophe Fremez Jean-Paul Motta Ayaka Shima Céline Besson Pauline Le Faouder Nicolas Cénac Marie-Paule Roth Hélène Coppin Maxime Fontanié Patricia Martin Jean-Philippe Nougayrède Eric Oswald Uropathogenic E. coli induces DNA damage in the bladder. PLoS Pathogens |
author_facet |
Camille V Chagneau Clémence Massip Nadège Bossuet-Greif Christophe Fremez Jean-Paul Motta Ayaka Shima Céline Besson Pauline Le Faouder Nicolas Cénac Marie-Paule Roth Hélène Coppin Maxime Fontanié Patricia Martin Jean-Philippe Nougayrède Eric Oswald |
author_sort |
Camille V Chagneau |
title |
Uropathogenic E. coli induces DNA damage in the bladder. |
title_short |
Uropathogenic E. coli induces DNA damage in the bladder. |
title_full |
Uropathogenic E. coli induces DNA damage in the bladder. |
title_fullStr |
Uropathogenic E. coli induces DNA damage in the bladder. |
title_full_unstemmed |
Uropathogenic E. coli induces DNA damage in the bladder. |
title_sort |
uropathogenic e. coli induces dna damage in the bladder. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Pathogens |
issn |
1553-7366 1553-7374 |
publishDate |
2021-02-01 |
description |
Urinary tract infections (UTIs) are among the most common outpatient infections, with a lifetime incidence of around 60% in women. We analysed urine samples from 223 patients with community-acquired UTIs and report the presence of the cleavage product released during the synthesis of colibactin, a bacterial genotoxin, in 55 of the samples examined. Uropathogenic Escherichia coli strains isolated from these patients, as well as the archetypal E. coli strain UTI89, were found to produce colibactin. In a murine model of UTI, the machinery producing colibactin was expressed during the early hours of the infection, when intracellular bacterial communities form. We observed extensive DNA damage both in umbrella and bladder progenitor cells. To the best of our knowledge this is the first report of colibactin production in UTIs in humans and its genotoxicity in bladder cells. |
url |
https://doi.org/10.1371/journal.ppat.1009310 |
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