<em>Trichoderma</em> spp. and <em>Bacillus subtilis</em> for control of <em>Dactylonectria macrodidyma</em> in grapevine

Black foot disease, which is primarily caused by Dactylonectria species, affects young and mature grapevines, causing loss of productivity and reduction of longevity of vineyards. Because it is a soil-borne fungus, this pathogen offers limited control possibilities. This study aimed to assess the b...

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Main Authors: Ricardo SANTOS, Leise HECKLER, Marília LAZAROTTO, Lucas GARRIDO, Cecilia REGO, Elena BLUME
Format: Article
Language:English
Published: Firenze University Press 2016-07-01
Series:Phytopathologia Mediterranea
Subjects:
Online Access:https://oajournals.fupress.net/index.php/pm/article/view/5669
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spelling doaj-89807536a7db4c7c99f61866bbbef8bd2020-11-25T03:51:02ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952016-07-0155210.14601/Phytopathol_Mediterr-1804815594<em>Trichoderma</em> spp. and <em>Bacillus subtilis</em> for control of <em>Dactylonectria macrodidyma</em> in grapevineRicardo SANTOS0Leise HECKLER1Marília LAZAROTTO2Lucas GARRIDO3Cecilia REGO4Elena BLUME5Escola Superior de Agricultura "Luiz de Queiroz"-ESALQ Universidade de São Paulo-USPUniversidade Federal de Santa MariaUniversidade Federal do Rio Grande do SulEmbrapa Uva e VinhoUniversidade de LisboaUniversidade Federal de Santa Maria Black foot disease, which is primarily caused by Dactylonectria species, affects young and mature grapevines, causing loss of productivity and reduction of longevity of vineyards. Because it is a soil-borne fungus, this pathogen offers limited control possibilities. This study aimed to assess the biocontrol activity of products based on Trichoderma spp. and Bacillus subtilis, in vitro and in vivo, against D. macrodidyma. In vitro assays were carried out to evaluate inhibition of mycelial growth of the pathogen through volatile metabolites and paired cultures assays. An in vivo experiment evaluated the potential biocontrol of the pathogen in plants of Vitis vinifera cv. Merlot, grafted on ‘Paulsen 1103’. In the volatile metabolites assay, mean inhibition of mycelial growth in treatments using Trichoderma spp. was 59%. In the paired cultures experiment, treatment with B. subtilis (Rizolyptus®) gave 41% inhibition of the pathogen. In the in vivo assay, the best results for length of the primary shoots, total numbers of nodes and root dry weight were achieved with the B. subtilis treatments. This study demonstrates that B. subtilis has potential for biocontrol of D. macrodidyma. https://oajournals.fupress.net/index.php/pm/article/view/5669Vitis viniferablack foot diseasesoil-borne pathogen
collection DOAJ
language English
format Article
sources DOAJ
author Ricardo SANTOS
Leise HECKLER
Marília LAZAROTTO
Lucas GARRIDO
Cecilia REGO
Elena BLUME
spellingShingle Ricardo SANTOS
Leise HECKLER
Marília LAZAROTTO
Lucas GARRIDO
Cecilia REGO
Elena BLUME
<em>Trichoderma</em> spp. and <em>Bacillus subtilis</em> for control of <em>Dactylonectria macrodidyma</em> in grapevine
Phytopathologia Mediterranea
Vitis vinifera
black foot disease
soil-borne pathogen
author_facet Ricardo SANTOS
Leise HECKLER
Marília LAZAROTTO
Lucas GARRIDO
Cecilia REGO
Elena BLUME
author_sort Ricardo SANTOS
title <em>Trichoderma</em> spp. and <em>Bacillus subtilis</em> for control of <em>Dactylonectria macrodidyma</em> in grapevine
title_short <em>Trichoderma</em> spp. and <em>Bacillus subtilis</em> for control of <em>Dactylonectria macrodidyma</em> in grapevine
title_full <em>Trichoderma</em> spp. and <em>Bacillus subtilis</em> for control of <em>Dactylonectria macrodidyma</em> in grapevine
title_fullStr <em>Trichoderma</em> spp. and <em>Bacillus subtilis</em> for control of <em>Dactylonectria macrodidyma</em> in grapevine
title_full_unstemmed <em>Trichoderma</em> spp. and <em>Bacillus subtilis</em> for control of <em>Dactylonectria macrodidyma</em> in grapevine
title_sort <em>trichoderma</em> spp. and <em>bacillus subtilis</em> for control of <em>dactylonectria macrodidyma</em> in grapevine
publisher Firenze University Press
series Phytopathologia Mediterranea
issn 0031-9465
1593-2095
publishDate 2016-07-01
description Black foot disease, which is primarily caused by Dactylonectria species, affects young and mature grapevines, causing loss of productivity and reduction of longevity of vineyards. Because it is a soil-borne fungus, this pathogen offers limited control possibilities. This study aimed to assess the biocontrol activity of products based on Trichoderma spp. and Bacillus subtilis, in vitro and in vivo, against D. macrodidyma. In vitro assays were carried out to evaluate inhibition of mycelial growth of the pathogen through volatile metabolites and paired cultures assays. An in vivo experiment evaluated the potential biocontrol of the pathogen in plants of Vitis vinifera cv. Merlot, grafted on ‘Paulsen 1103’. In the volatile metabolites assay, mean inhibition of mycelial growth in treatments using Trichoderma spp. was 59%. In the paired cultures experiment, treatment with B. subtilis (Rizolyptus®) gave 41% inhibition of the pathogen. In the in vivo assay, the best results for length of the primary shoots, total numbers of nodes and root dry weight were achieved with the B. subtilis treatments. This study demonstrates that B. subtilis has potential for biocontrol of D. macrodidyma.
topic Vitis vinifera
black foot disease
soil-borne pathogen
url https://oajournals.fupress.net/index.php/pm/article/view/5669
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