Utilization of shrimp wastewater for poly-β-hydroxybutyrate production by Synechocystis sp. PCC 6803 strain ΔSphU cultivated in photobioreactor

• Main Authors: Nattawut Krasaesueb, Aran Incharoensakdi, Wanthanee Khetkorn
• Format: Article
• Language: English
• Published: Elsevier 2019-09-01
• Series: Biotechnology Reports
• Online Access: http://www.sciencedirect.com/science/article/pii/S2215017X1930030X

The wastewater discharge from the intensive shrimp aquaculture contains high concentration of nutrients, which can lead to eutrophication. This study aimed to reuse the shrimp wastewater for low cost cyanobacterial cultivation to produce biodegradable plastic poly-β-hydroxybutyrate (PHB). The Synechocystis sp. PCC 6803 (ΔSphU) lacking phosphate regulator (SphU) could utilize nutrients in shrimp wastewater for promoting biomass yield of 500 mg L−1 after 14 days. The ΔSphU showed the highest phosphate uptake rate of 20.16 mggDw−1d−1 at the first day of photobioreactor running. In addition, the nutrient removal efficiencies were 96.99% for phosphate, 80.10% for nitrate, 67.90% for nitrite and 98.07% for ammonium. The reduction of nitrate in shrimp wastewater due to nitrogen assimilation could induce PHB accumulation in ΔSphU. The highest PHB content was 32.48% (w/w) DW, with the maximum PHB productivity of 12.73 mg L−1d−1. The produced PHB of ΔSphU had material properties similar to those of the commercial PHB. Keywords: Shrimp wastewater, Batch photobioreactor, Synechocystis sp. PCC 6803 strain ΔSphU, Poly-β-hydroxybutryate, Nutrient remediation