Medicinal Plant Polyphenols Attenuate Oxidative Stress and Improve Inflammatory and Vasoactive Markers in Cerebral Endothelial Cells during Hyperglycemic Condition

Blood-brain barrier endothelial cells are the main targets of diabetes-related hyperglycemia that alters endothelial functions and brain homeostasis. Hyperglycemia-mediated oxidative stress may play a causal role. This study evaluated the protective effects of characterized polyphenol-rich medicinal...

Full description

Bibliographic Details
Main Authors: Janice Taïlé, Angélique Arcambal, Patricia Clerc, Anne Gauvin-Bialecki, Marie-Paule Gonthier
Format: Article
Language:English
Published: MDPI AG 2020-07-01
Series:Antioxidants
Subjects:
Online Access:https://www.mdpi.com/2076-3921/9/7/573
id doaj-896b45d6bdf44e7cb83f778990ef918c
record_format Article
spelling doaj-896b45d6bdf44e7cb83f778990ef918c2020-11-25T03:06:46ZengMDPI AGAntioxidants2076-39212020-07-01957357310.3390/antiox9070573Medicinal Plant Polyphenols Attenuate Oxidative Stress and Improve Inflammatory and Vasoactive Markers in Cerebral Endothelial Cells during Hyperglycemic ConditionJanice Taïlé0Angélique Arcambal1Patricia Clerc2Anne Gauvin-Bialecki3Marie-Paule Gonthier4Diabète athérothrombose Thérapies Réunion Océan Indien, INSERM, UMR 1188, Université de La Réunion, 2 rue Maxime Rivière, 97490 Sainte-Clotilde, La Réunion, FranceDiabète athérothrombose Thérapies Réunion Océan Indien, INSERM, UMR 1188, Université de La Réunion, 2 rue Maxime Rivière, 97490 Sainte-Clotilde, La Réunion, FranceLaboratoire de Chimie et de Biotechnologie des Produits Naturels, Université de La Réunion, UR 2212, 15 Avenue René Cassin CS 92003, 97744 Saint-Denis CEDEX 9, La Réunion, FranceLaboratoire de Chimie et de Biotechnologie des Produits Naturels, Université de La Réunion, UR 2212, 15 Avenue René Cassin CS 92003, 97744 Saint-Denis CEDEX 9, La Réunion, FranceDiabète athérothrombose Thérapies Réunion Océan Indien, INSERM, UMR 1188, Université de La Réunion, 2 rue Maxime Rivière, 97490 Sainte-Clotilde, La Réunion, FranceBlood-brain barrier endothelial cells are the main targets of diabetes-related hyperglycemia that alters endothelial functions and brain homeostasis. Hyperglycemia-mediated oxidative stress may play a causal role. This study evaluated the protective effects of characterized polyphenol-rich medicinal plant extracts on redox, inflammatory and vasoactive markers on murine bEnd3 cerebral endothelial cells exposed to high glucose concentration. The results show that hyperglycemic condition promoted oxidative stress through increased reactive oxygen species (ROS) levels, deregulated antioxidant superoxide dismutase (SOD) activity, and altered expression of genes encoding Cu/ZnSOD, MnSOD, catalase, glutathione peroxidase (GPx), heme oxygenase-1 (HO-1), NADPH oxidase 4 (Nox4), and nuclear factor erythroid 2-related factor 2 (Nrf2) redox factors. Cell preconditioning with inhibitors of signaling pathways highlights a causal role of nuclear factor kappa B (NFκB), while a protective action of AMP-activated protein kinase (AMPK) on redox changes. The hyperglycemic condition induced a pro-inflammatory response by elevating NFκB gene expression and interleukin-6 (IL-6) secretion, and deregulated the production of endothelin-1 (ET-1), endothelial nitric oxide synthase (eNOS), and nitric oxide (NO) vasoactive markers. Importantly, polyphenolic extracts from <i>Antirhea borbonica</i>, <i>Ayapana triplinervis</i>, <i>Dodonaea viscosa</i>, and <i>Terminalia bentzoe</i> French medicinal plants, counteracted high glucose deleterious effects by exhibiting antioxidant and anti-inflammatory properties. In an innovative way, quercetin, caffeic, chlorogenic and gallic acids identified as predominant plant polyphenols, and six related circulating metabolites were found to exert similar benefits. Collectively, these findings demonstrate polyphenol protective action on cerebral endothelial cells during hyperglycemic condition.https://www.mdpi.com/2076-3921/9/7/573cerebral endothelial cellshyperglycemiaoxidative stressinflammationantioxidant plant polyphenols
collection DOAJ
language English
format Article
sources DOAJ
author Janice Taïlé
Angélique Arcambal
Patricia Clerc
Anne Gauvin-Bialecki
Marie-Paule Gonthier
spellingShingle Janice Taïlé
Angélique Arcambal
Patricia Clerc
Anne Gauvin-Bialecki
Marie-Paule Gonthier
Medicinal Plant Polyphenols Attenuate Oxidative Stress and Improve Inflammatory and Vasoactive Markers in Cerebral Endothelial Cells during Hyperglycemic Condition
Antioxidants
cerebral endothelial cells
hyperglycemia
oxidative stress
inflammation
antioxidant plant polyphenols
author_facet Janice Taïlé
Angélique Arcambal
Patricia Clerc
Anne Gauvin-Bialecki
Marie-Paule Gonthier
author_sort Janice Taïlé
title Medicinal Plant Polyphenols Attenuate Oxidative Stress and Improve Inflammatory and Vasoactive Markers in Cerebral Endothelial Cells during Hyperglycemic Condition
title_short Medicinal Plant Polyphenols Attenuate Oxidative Stress and Improve Inflammatory and Vasoactive Markers in Cerebral Endothelial Cells during Hyperglycemic Condition
title_full Medicinal Plant Polyphenols Attenuate Oxidative Stress and Improve Inflammatory and Vasoactive Markers in Cerebral Endothelial Cells during Hyperglycemic Condition
title_fullStr Medicinal Plant Polyphenols Attenuate Oxidative Stress and Improve Inflammatory and Vasoactive Markers in Cerebral Endothelial Cells during Hyperglycemic Condition
title_full_unstemmed Medicinal Plant Polyphenols Attenuate Oxidative Stress and Improve Inflammatory and Vasoactive Markers in Cerebral Endothelial Cells during Hyperglycemic Condition
title_sort medicinal plant polyphenols attenuate oxidative stress and improve inflammatory and vasoactive markers in cerebral endothelial cells during hyperglycemic condition
publisher MDPI AG
series Antioxidants
issn 2076-3921
publishDate 2020-07-01
description Blood-brain barrier endothelial cells are the main targets of diabetes-related hyperglycemia that alters endothelial functions and brain homeostasis. Hyperglycemia-mediated oxidative stress may play a causal role. This study evaluated the protective effects of characterized polyphenol-rich medicinal plant extracts on redox, inflammatory and vasoactive markers on murine bEnd3 cerebral endothelial cells exposed to high glucose concentration. The results show that hyperglycemic condition promoted oxidative stress through increased reactive oxygen species (ROS) levels, deregulated antioxidant superoxide dismutase (SOD) activity, and altered expression of genes encoding Cu/ZnSOD, MnSOD, catalase, glutathione peroxidase (GPx), heme oxygenase-1 (HO-1), NADPH oxidase 4 (Nox4), and nuclear factor erythroid 2-related factor 2 (Nrf2) redox factors. Cell preconditioning with inhibitors of signaling pathways highlights a causal role of nuclear factor kappa B (NFκB), while a protective action of AMP-activated protein kinase (AMPK) on redox changes. The hyperglycemic condition induced a pro-inflammatory response by elevating NFκB gene expression and interleukin-6 (IL-6) secretion, and deregulated the production of endothelin-1 (ET-1), endothelial nitric oxide synthase (eNOS), and nitric oxide (NO) vasoactive markers. Importantly, polyphenolic extracts from <i>Antirhea borbonica</i>, <i>Ayapana triplinervis</i>, <i>Dodonaea viscosa</i>, and <i>Terminalia bentzoe</i> French medicinal plants, counteracted high glucose deleterious effects by exhibiting antioxidant and anti-inflammatory properties. In an innovative way, quercetin, caffeic, chlorogenic and gallic acids identified as predominant plant polyphenols, and six related circulating metabolites were found to exert similar benefits. Collectively, these findings demonstrate polyphenol protective action on cerebral endothelial cells during hyperglycemic condition.
topic cerebral endothelial cells
hyperglycemia
oxidative stress
inflammation
antioxidant plant polyphenols
url https://www.mdpi.com/2076-3921/9/7/573
work_keys_str_mv AT janicetaile medicinalplantpolyphenolsattenuateoxidativestressandimproveinflammatoryandvasoactivemarkersincerebralendothelialcellsduringhyperglycemiccondition
AT angeliquearcambal medicinalplantpolyphenolsattenuateoxidativestressandimproveinflammatoryandvasoactivemarkersincerebralendothelialcellsduringhyperglycemiccondition
AT patriciaclerc medicinalplantpolyphenolsattenuateoxidativestressandimproveinflammatoryandvasoactivemarkersincerebralendothelialcellsduringhyperglycemiccondition
AT annegauvinbialecki medicinalplantpolyphenolsattenuateoxidativestressandimproveinflammatoryandvasoactivemarkersincerebralendothelialcellsduringhyperglycemiccondition
AT mariepaulegonthier medicinalplantpolyphenolsattenuateoxidativestressandimproveinflammatoryandvasoactivemarkersincerebralendothelialcellsduringhyperglycemiccondition
_version_ 1724672553878814720